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香兰素衍生物VND3207对小鼠骨髓细胞遗传损伤的防护效应研究 被引量:2

Radiological protection effect of vanillin derivative VND3207 against radiation-induced cytogenetic damage in mouse bone marrow cells
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摘要 目的 研究香兰素衍生物4-羟基-3,5-二甲氧基苯甲醛(VND3207)对γ射线整体照射小鼠骨髓细胞遗传损伤的防护作用.方法 BALB/c小鼠按10、50和100 mg/kg不同剂量灌胃给药,1次/d,连续5 d,最后一次给药后2 h,2 Gy60Co γ射线照射,照射后48 h观察小鼠骨髓嗜多染红细胞微核、染色体畸变和骨髓有丝分裂指数的改变.结果 不同剂量(10、50和100 mg/kg)的VND3207均能有效地降低小鼠骨髓嗜多染红细胞微核率(t=2.40~4.26,P<0.05)和染色体畸变率(t=2.36~3.52,P<0.05),同时提高骨髓有丝分裂指数.药物保护效果与给药剂量呈依赖关系,其中100 mg/kg剂量组的微核率和染色体畸变率与单纯照射组相比,分别降低了65%和50%.结论 VND3207对60Co γ射线诱发的小鼠骨髓辐射损伤有良好的保护作用. Objective To study the protection of vanillin derivative VND3207 on the cytogenetic damage of mouse bone marrow cell induced by ionizing radiation.Methods BALB/c mice were randomly divided into five groups:normal control group,2 Gy dose irradiation group,and three groups of 2 Gy irradiaiton with VND3207 protection at doses of 10,50 and 100 mg/kg,respectively.VND3207 was given by intragastric administration once a day for five days.Two hours after the last drug administration,the mice were irradiated with 2 Gy γ-rays.The changes of polychromatophilic erythroblasts micronuclei (MN),chromosome aberration (CA) and mitosis index (MI) of mouse bone marrow cells were observed at 24 and 48 h after irradiation.Results Under the protection of VND3207 at the dosages 10,50,100 mg/kg,the yields of poly-chromatophilic erythroblasts MN and CA of bone marrow cells were significantly decreased(t = 2.36-4.26,P < 0.05),and the marrow cells MI remained much higher level compared with the irradiated mice without drug protection (t = 2.58,2.01,P < 0.05).The radiological protection effect was drug dose-dependent,and the administration of VND3207 at the dosage of 100 mg/kg resulted in reduction by 50% and 65% in the yields of MN and CA,respectively.Conclusions VND3207 had a good protection effect of on γ-ray induced cytogentic damage of mouse bone marrow cells.
作者 汪传高 汪黎 周平坤 王仲文 胡永哲 靳海明 张学清 陈英 WANG Chuan-gao,WANG Li,ZHOU Ping-kun,WANG Zhong-wen,HU Yong-zhe,JIN Hai-ming,ZHANG Xue-qing,CHEN Ying( 1.Department of Radiation Toxicology and Oncology,Beijing Institute of Radiation Medicine,Beijing 100850,China;)
出处 《中华放射医学与防护杂志》 CAS CSCD 北大核心 2010年第5期558-560,共3页 Chinese Journal of Radiological Medicine and Protection
基金 国家自然科学基金杰出青年基金(C30825011)
关键词 香兰素衍生物 Γ射线 嗜多染红细胞微核 染色体畸变 有丝分裂指数 辐射防护剂 Vanillin derivative γ-ray Polychromatophilic erythroblasts micronuclei Chromosome aberration Mitotic index Radioprotective agent
作者简介 通信作者:陈英,Email:yingchen29@yahoo.con.cn
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