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羟基红花黄色素A对人胃癌移植瘤裸鼠瘤组织bFGF蛋白及MMP-9表达的影响 被引量:14

Effects of HSYA on expression of bFGF protein and MMP-9 in BGC-823 transplantation tumor of nude mice
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摘要 目的:研究羟基红花黄色素A(hydroxy safflor yellow A,HSYA)对人胃癌裸鼠皮下移植瘤碱性成纤维生长因子(basic fibroblast growth factor,bFGF)蛋白以及基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)mRNA及蛋白表达的影响。方法:采用BALB/C nu/nu裸小鼠接种人胃腺癌细胞株BGC-823右前肢腋部皮下建立裸鼠人癌移植瘤模型,随机分为模型组、阳性对照环磷酰胺组(2 g·L^-1)、HSYA组(0.056,0.028 g·L^-1),观察抑瘤作用,并采用实时荧光定量PCR(real time-fluorescent quantitation PCR,RTFQ-PCR)检测瘤组织中MMP-9 mRNA表达;酶联免疫吸附测定法(enzyme linked immunosorbentassay,ELISA)检测瘤组织中bFGF,MMP-9蛋白表达。结果:HSYA0.028 g·L^-1组抑瘤效果明显,且其MMP-9 mRNA表达及bFGF与MMP-9蛋白表达与模型组比,均明显降低(P〈0.05)。结论:一定浓度的HSYA抑制肿瘤生长的机制之一可能与抑制bFGF,MMP-9的表达,减少瘤组织血管基底膜的降解,阻抑血管移行以减少血管生成有关。 Objective: To investigate the effects of hydroxy safflor yellow A(HSYA) on the expression of bFGF protein and MMP-9 mRNA or protein of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice.Method: The BGC-823 cells were subcutaneouly injected into the right anterior armpit of BALB/C nu/nu nude mice,and the animal model of transplantation tumor was established.The experimental groups were treated with HSYA at concentration of 0.056 and 0.028 g·L^-1and cyclophosphamide at 2 g·L^-1or with physiologic saline.The tumor inhibitory effect was observed,and the mRNA expression of MMP-9 of transplantation tumor was detected by real time-fluorescent quantitation PCR and the protein expression of MMP-9 and bFGF were detected by enzyme linked immunosorbent assay.Result: The IR in the group with HSYA at the concentration of 0.028 g·L^-1is higher than in the group with normal sodium.After treatment with HSYA,the mRNA expression of MMP-9 has significant difference at the concentration of 0.028 g·L^-1as compared with physiologic saline-treated group(P〈0.05),but the protein expression of MMP-9 and bFGF is obviously less than that in the physiologic saline-treated group(P〈0.05).Conclusion: The possible mechanism of HSYA in given concentration to antagonize tumor angiogenesis may be related with inhibiting the protein expression of MMP-9 and bFGF or the mRNA expression of MMP-9 in tumor tissue to reduce the degradation of blood vessel basilar membrane,and to restrain the migration of blood vessel and decrease the tumor vascularization.
作者 奚胜艳 张前 刘朝阳 解华 岳利峰 赵育芳 臧宝霞 高学敏 XI Shengyan , ZHANG Qian , LIU Chaoyang, XIE Hua, YUE Lifeng , ZHAO Ytffang , ZANG Baoxia, GAO Xuemin (1. Department of Traditional Chinese Medicine of Medical College of Xiamen University, Xiamen 361005, China; 2. Tumor Research Center of Cancer Hospital of Chinese Academy of Medical Sciences, Beijing 100021, China; 3. Beijing University of Chinese Medicine, Beijing 100029, China; 4. Beijing Institute of Cardiopulmonary Vascular Disease, Beijing 100029, China)
出处 《中国中药杂志》 CAS CSCD 北大核心 2010年第21期2877-2881,共5页 China Journal of Chinese Materia Medica
基金 国家自然科学基金项目(30572436)
关键词 羟基红花黄色素A BGC-823 碱性成纤维生长因子 基质金属蛋白酶9 肿瘤血管生成 hydroxy safflor yellow A BGC-823 bFGF MMP-9 tumor angiogenesis
作者简介 奚胜艳,医学博士,讲师,Tel:13616043852,E-mail:xishengyan13204@163.com [通信作者]张前,Tel:(010)64287017,E-mail:zql9881988@163.com
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