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中等强度恒定磁场作用下的神经元钠通道特性 预览 被引量:6

Characteristics of Neurons Sodium Channel Under Moderate Intensity Static Magnetic Field
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摘要 为了从离子通道的角度研究中等强度恒定磁场(静磁场)对神经细胞的影响,利用线圈及磁铁产生3 mT、30 mT和100 mT的恒定磁场,作用于急性分离的小鼠前额叶皮层锥体神经元,应用全细胞膜片钳技术精密测量其钠通道的电流情况.实验发现:不同中等强度的恒定磁场均使神经细胞钠通道激活电位向超极化方向移动,钠电流峰值随磁场强度、暴露时间的改变而不同程度地增大.100 mT恒定磁场作用可改变激活和失活过程,使激活/失活曲线的半数激活/失活电压和斜率因子发生变化(激活曲线的半数激活电压由(-33.73±0.42)mV变为(-37.06±1.03)mV,斜率因子由(4.19±0.32)mV变为(6.79±0.93)mV,而失活曲线的半数失活电压由(-54.35±0.46)mV变为(-51.43±0.36)mV,斜率因子由(6.54±0.40)mV变为(7.31±0.31)mV).结果表明,3 mT、30 mT和100mT恒定磁场均可改变神经元钠通道特性,从而影响神经元的生理功能.本实验为深入探讨恒定磁场对细胞离子通道的影响与作用提供了一定的实验基础. From the perspective of ion channels,the effect of moderate intensity static magnetic field(SMF) on neural cells was investigated.Acutely isolated rat prefrontal cortex pyramidal neurons were exposed to 3 mT,30 mT and 100 mT SMF,respectively,generated by a coil and a magnet,and the current of sodium channel was precisely measured using the whole-cell patch clamp technique.The experiment revealed that the activation potential of sodium ion channel shifted towards the hyperpolarization in all of the three moderate intensity SMFs,and the increase of the amplitudes of sodium ion channel current varied with the intensity and exposure time of the magnetic field.The 100 mT SMF exposure affected the activation and inactivation process of sodium ion channel current,and changed the half-activation/inactivation voltage and slope factor of the activation/inactivation curves.(The half-activation voltage of the activation curves changed from(-33.73±0.42) mV to(-37.06±1.03) mV and the slope factor of the activation curves changed from(4.19±0.32) mV to(6.79±0.93) mV,and the half-inactivation voltage of the inactivation curves changed from(-54.35±0.46) mV to(-51.43±0.36) mV and the slope factor of the inactivation curves changes from(6.54±0.40) mV to(7.31±0.31) mV).Experimental results show that 3 mT,30 mT and 100 mT SMFs can change the characteristics of voltage-gated sodium channel,and further adjust the physiological functions of the neurons,which serves as the experimental basis for further research into the effect of SMF on cell ion channels.
作者 程立君 李刚 林凌 乔晓艳 曾锐利 张宝菊 佟颖 CHENG Li-jun,LI Gang,LIN Ling,QIAO Xiao-yan,ZENG Rui-li,ZHANG Bao-ju,TONG Ying,(1.Tianjin Key Laboratory of Biomedical Detecting Techniques and Instruments,Tianjin University,Tianjin 300072,China;2.College of Physics & Electronics Engineering,Shanxi University,Taiyuan 030006,China;3.Department of Automobile Engineering,Academy of Military Transportation,Tianjin 300161,China;4.College of Physics and Electronic Information,Tianjin Normal University,Tianjin 300387,China)
出处 《纳米技术与精密工程》 EI CAS CSCD 2010年第6期 559-564,共6页 Nanotechnology and Precision Engineering
基金 天津市应用基础及前沿技术研究计划资助项目(08JCZDJC19400)
关键词 静磁场 钠离子通道 膜片钳技术 生物刺激效应 static magnetic field sodium ion channel patch clamp technique biological effect
作者简介 程立君(1981-),女,博士. 通讯作者:佟颖,chendiun1220@yahoo.com.cn
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  • 1Pang L J, Baciu C, Traitcheva N, et al. Photodynamic effect on cancer cells influenced by electromagnetic fields [ J]. Journal of Photochemistry and Photobiology B:Biology, 2001,64(1): 21-26. 被引量:1
  • 2Thomas A W, White K P, Drost D J, et al. A comparison of rheumatoid arthritis and fibromyalgia patients and healthy controls exposed to a pulsed (200 μT) magnetic field: Effects on normal standing balance [ J ]. Neuroscience Letters, 2001, 309( 1 ) : 17-20. 被引量:1
  • 3Shupak N M, Prato F S, Thomas A N. Human exposure to a specific pulsed magnetic field: Effects on thermal sensory and pain thresholds [ J ]. Neuroscience Letters, 2004, 363 (2) : 157-162. 被引量:1
  • 4Shupak N M, Hensel J M, Cross-Mellor S K, et al. Analgesic and behavioral effects of a 100 μT specific pulsed extremely low frequency magnetic field on control and morphine treated CF-1 mice [ J ]. Neuroscience Letters, 2004, 354( 1 ) : 30-33. 被引量:1
  • 5周取英,叶小兰,李小芒.恒定磁场治疗急性特发性面神经炎的疗效分析与护理[J].护理实践与研究,2008,5(10):31-32. 被引量:1
  • 6张杰,李振华,孙晋浩,暴丽华,刘岳鹏.恒定磁场对Schwann细胞氧化损伤的保护作用[J].山东大学学报:医学版,2007,45(3):229-232. 被引量:5
  • 7Yawara E, Marl O I, Shoogo U. Control of orientation of rat Schwann cells using an 8-T static magnetic field [ J ]. Neuroscience Letters, 2003, 351(2) : 130-132. 被引量:1
  • 8Pacini S, Vannelli G B, Barni T, et al. Effect of 0. 2 T static magnetic field on human neurons: Remodeling and inhibition of signal transduction without genome instability [ J ]. Neuroscience Letters, 1999, 267 ( 3 ) : 185-188. 被引量:1
  • 9姜虹,贾忠葆,贾洁虹,唐桂芳,韩秀筠.浅谈磁疗的作用[J].生物磁学,2003,3(1):37-37. 被引量:3
  • 10李刚,程立君,林凌,贾方荣.小鼠海马神经元急性分离和膜片钳技术的应用[J].天津大学学报,2008,41(10):1157-1161. 被引量:11

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