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7个杏李品种花粉SFB基因的克隆鉴定 预览 被引量:6

Identification of SFB genes of 7 Prunus simonii
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摘要 以美国杏李品种为试验材料,利用Prunus的SFB基因特异性引物,对7个杏李品种进行PCR扩增,并进行克隆、测序。采用生物信息学分析方法分离鉴定了‘恐龙蛋’等7个杏李品种的花粉SFB基因。PCR结果显示,‘风味皇后’、‘味帝’、‘味王’、‘风味玫瑰’、‘味厚’、‘味馨’、‘恐龙蛋’等7个品种均扩增出一条460 bp左右的片段;对PCR产物回收克隆后测序,并通过BLAST比对和生物信息学分析表明,‘风味皇后’、‘味王’、‘风味玫瑰’、‘味厚’、‘味帝’、‘恐龙蛋’等6个品种中克隆到了一个SFBh基因;‘风味皇后’、‘味王’、‘风味玫瑰’、‘味厚’、‘味馨’等5个品种中克隆到了一个SFBe基因;‘味帝’和‘恐龙蛋’中分离克隆了SFBb基因;‘味馨’中分离克隆了一个SFB2基因;故7个杏李品种的花粉SFB基因型鉴定为:‘风味皇后’、‘味王’、‘风味玫瑰’和‘味厚’等4个品种的花粉SFB基因型相同,为SFBeSFBh;‘味帝’和‘恐龙蛋’的花粉SFB基因型相同,为SFBbSFBh;味馨’的花粉SFB基因型为SFBeSFB2。 Introduction of American plums was used as the experimental materials.Adopting SFB specific primer in Prunus,PCR amplification,cloning,sequencing of 7 Prunus simonii were carried out,and bioinformatics analysis method was adopted for isolation and identification of the SFB genes of 7 Prunus simonii.The resutts of PCR amplification show that a common fragment of about 460 bp was generated in 'Fengweihuanghou','Weidi','Weiwang','Fengweimeigui','Weihou','Weixin' and 'Konglongdan';DNA sequence bioinformatic analysis and BLAST reveal that a SFBh gene was separated from 'Fengweihuanghou','Weiwang','Fengweimeigui','Weihou','Weixin','Konglongdan';a SFBe gene was separated from 'Fengweihuanghou','Weiwang','Fengweimeigui','Weihou','Weixin';a SFBb gene was separated from 'Weidi' and 'Konglongdan';a SFB2 gene was separated from 'Weixin'.The SFB-genotypes of 7 Prunus Simonii as follows:'Fengweihuanghou(SFBeSFBh)','Weiwang(SFBeSFBh)','Fengweimeigui(SFBeSFBh)','Weihou(SFBeSFBh)','Weidi(SFBbSFBh)','Konglongdan(SFBbSFBh)','Weixin(SFBeSFB2)'.
作者 李芳东 乌云塔娜 杜红岩 李洪果 杨绍彬 LI Fang-dong,WUYUN Ta-na,DU Hong-yan,LI Hong-guo,YANG Shao-bin(1.Beijing Forestry University,Beijing 100083,China;2.Non-timber Forest Research and Development Center of Chinese Academy of Forestry,Zhengzhou 450003,Henan,China;3.The Key Lab of Non-wood Forest Product of Forestry Ministry,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;4.School of Forestry,Central South University of Forestry and Technology,Changsha 410004,Hunan,China)
出处 《中南林业科技大学学报:自然科学版》 CAS CSCD 北大核心 2010年第12期 1-5,共5页 Journal of Central South Forestry University
基金 国家”十一五”科技支撑项目“名特优经济林优质高产培育技术”(2006BAD18B02)
关键词 杏李 自交不亲和性 F-BOX SFB基因 Prunus domestica ×armeniaca self-incompatibility F-Box SFB gene
作者简介 作者简介:李芳东(1963-),男,河南太康人,博士后,研究员。主要从事经济林育种与栽培的研究;Email:lifangdong66@126.com
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