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云南省思茅地区乙型脑炎病毒及黄热病毒的分子流行病学监测 预览 被引量:5

Molecular Epidemiological Surveillance of YEV and YFV in Simao of Yunnan
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摘要 2009年至2010年,在云南省思茅地区普文镇、思茅区、震东乡、六顺乡、勐旺乡等地共采集17880只蚊子(分为91管),其中库蚊16500只(83管),按蚊1380只(8管)。采用RT-PCR方法,对91管蚊虫样本进行流行性乙型脑炎病毒NS1基因及黄热病毒E基因片段扩增,回收阳性PCR产物,并进行测序和遗传进化分析。结果表明,思茅地区库蚊乙型脑炎病毒检测阳性率为18.1%(15管/83管),黄热病毒检测阳性率为15.7%(13管/83管),思茅地区按蚊乙型脑炎病毒检测阳性率为25%(2管/8管),黄热病毒检测阳性率为12.5%(1管/8管)。克隆的流行性乙型脑炎病毒NS1基因及黄热病毒E基因与JEV和YFV参考株进行序列比对,与乙型脑炎病毒参考株同源性为88.3%-90.7%。监测结果显示,思茅地区蚊子JEV和YFv带毒率均较高,表明当地两种虫媒病毒病流行风险高。 From 2009 to 2010, 17 880 mosquitoes (divided into 91tubes) were collected from Puwen Town, Simao District, Zhendong Town, Liushun Town, and Mengwang Town of Simao in Yunnan Province, among which Culex and Anopheles were 16 500 (83) and 1 380 (8) respectively. NS1 gene of Japanese encephalitis virus and E gene of yellow fever virus were amplified by RT-PCR, and then positive PCR products were recovered, sequenced and phylogenetically analysised. The results showed that in Culex the proportion of JEV-positive was 18.1% (15/83), the proportion of YFV-positive was 15.7% (13/83), in Anopheles JEV-positive rate was 25%(2/8), and yellow fever virus-positive rate was 12.5% (1/8). Compared cloned NS1 gene of Japanese encephalitis virus and E gene of yellow fever virus with reference strains of JEV and YFV, the homology rate was about 88.3%-90.7%. The results showed that infected JEV and YFV mosquitoes rate were high in Simao region, which indicated that epidemic risks of two local insect-borne virus disease were high.
作者 刘振江 金宁一 鲁会军 李国江 刘昊 岳云强 郭欢欢 周红宁 杨中华 谢辉 Liu Zhenjiang,Jin Ningyi,Lu Huijun,Li Guojiang,Liu Hao,Yue Yunqiang, Guo Huanhuan,Zhou Hongning, Yang Zhonghuaa, Xie Hui(1. College of Animal Science, Jilin Agricultural University, Changchun 130118, China; 2.Institute of Military Veterinary, A- cademy of Military Medical Sciences, Changchun 130062, China; 3.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;4.Yunnan Institute of Parasitic Diseases,Simao 665000, China; 5.Jilin Agricaltural and Technology College, Jilin 132101, China)
出处 《中国动物检疫》 CAS 2011年第3期 65-67,77,共4页 China Journal Of Animal Quarantine
基金 基金项目:国家公益性行业科研专项项目(200803015) “863”项目(2009AA10Z20)
关键词 乙型脑炎病毒 黄热病毒 RT-PCR 检测 Japanese encephalitis virus: yellow fever virus: RT-PCR detection
作者简介 通讯作者:金宁一
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