期刊文献+

不同菌源诱导免疫的黄粉虫抗菌肽提取物对26种病原菌抑菌活性分析 预览 被引量:2

Analysis of the Antimicrobial Activity of Antimicrobial Peptides Extracted from Immunized Tenebrio molitor Induced by Different Microbial Treatments Against 26 Pathogens
在线阅读 下载PDF
收藏 分享 导出
摘要 [目的]探索经不同菌源诱导黄粉虫所产生的免疫物质——抗菌肽对病原菌是否存在活性差异。[方法]对饥饿后黄粉虫幼虫通过饲细菌、针刺饲真菌(放线菌)法产生诱导免疫反应,研磨、冷冻离心法获得抗菌肽提取物,采用抑菌圈法测定其对26种病原菌抑菌活性,利用三等分法划分四种类型评价抑菌活性及抑菌谱。[结果]经不同菌源诱导黄粉虫所产生的抗菌肽抑菌活性及抑菌谱上均存在明显差异。[结论]生防菌源在诱导昆虫体液免疫物质——抗菌肽表达上具有明显优势。 [Objective] The study was to explore whether antimicrobial activity of the antimicrobial peptides extracted from immunized Tenebrio molitor varied with different pathogens as inducers.[Method]By inducing T.molitor hungry larvaes to generate immune response via feeding with bacteria and with fungi or actinomycete post to pricking,the antimicrobial peptides extracts were obtained by grinding and centrifuging the cultures.Its antimicrobial activity against 26 pathogens was measured by bacteriostatic ring,and evaluated by trisection to four types and inhibitory spectrum.[Result]Both the antimicrobial activity and antimicrobial spectrum of the antimicrobial peptides extracts varied remarkably among different pathogens as inducers.[Conclusion]Bio-control used strains have obvious advantage in inducing the insect to express body fluid immunity material-antimicrobial peptides.
作者 谢咸升 董建臻 李静 李永利 王圆圆 李小凤 张亚南 XIE Xian-sheng1,2,DONG Jian-zhen2,LI Jing2,LI Yong-li2,WANG Yuan-yuan2,LI Xiao-feng2,ZHANG Ya-nan21.Wheat Research Institute,Shanxi Academy of Agricultural Sciences,Linfen 041000;2.College of Plant Protection,Agricultural University of Hebei,Baoding 071001
出处 《农业科学与技术:英文版》 CAS 2010年第9期 1-4,9,共5页 Agricultural Science & Technology
基金 河北省自然科学基金项目(C200700450).
关键词 黄粉虫 诱导免疫反应 抗菌肽 抑菌活性 抑菌谱 Tenebrio molitor Induced immune response Antimicrobial peptides Antimicrobial activity Antimicrobial spectrum
作者简介 谢咸升(1973-)男,山西临猗人,硕士研究生,助理研究员,从事昆虫免疫及生物防治研究,E-mail:xxshlf@163.com。*通讯作者。
  • 相关文献

参考文献15

二级参考文献160

共引文献100

同被引文献27

引证文献2

二级引证文献7

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈