期刊文献+

磷酸氯喹对白血病细胞株的影响 预览 被引量:1

The effect of chloroquine phosphate on leukemia cell lines
在线阅读 下载PDF
分享 导出
摘要 目的本实验以药物磷酸氯喹干预白血病细胞株,观察其对白血病细胞生长凋亡的影响,同时研究用药后凋亡相关蛋白Pnas-2的变化,以探寻磷酸氯喹作用白血病细胞株机制。方法 MTT法检测药物干预后白血病细胞增长情况;AnnexinⅤ/sytox标记细胞以流式细胞仪检测药物干预前后细胞凋亡情况的差别;激光共聚焦观察蛋白Pnas-2的亚细胞定位,蛋白印迹实验检测药物作用前后,Pnas-2表达量的变化。结果 50μg/mL的磷酸氯喹能够诱导白血病细胞株凋亡。并发现凋亡相关蛋白Pnas-2在白血病细胞株中存在异常定位和过量表达。磷酸氯喹能够使白血病细胞株中Pnas-2蛋白的表达量及亚细胞定位发生变化。结论磷酸氯喹能够抑制白血病细胞株生长,诱导凋亡,机制可能是恢复了Pnas-2蛋白的异常定位及表达。 Objective In this study,we treated leukemia cell lines with chloroquine phosphate to observe its effect on the cell lines.Besides we explored changes of the protein Pnas-2 after the intervention of the drug,in order to found the mechanism of the drug that influenced leukemia cell lines.Methods Chloroquine phosphate of defferent concentrations was added into the culture fluid at logarithmic phase.MTT assay was used to measure the cell proliferation,flow cytometry was applied to detect the cell apoptosis,immunofluorescence technology was employed to observe the subcellular location of protein Pnas-2,and Western blot was used to compare the expression content of Pnas-2.Results 50 μg/mL chloroquine phosphate could induce leukemic cell lines to apoptosis.And in leukemic cell lines,protein Pnas-2's expression content was more than non-tumor cell lines,and its subcellular location was abnormal,too.Simultaneously,the drug could revert Pnas-2's abnormal subcellular location and its redundant expression content.Conclusion chloroquine phosphate could inhibit the growth of leukemic cell lines and induce apoptosis.The mechanism may be related to its reversion of Pnas-2's abnormal content and location.
作者 刘佳 陈芳源 王海嵘 钟华 钟济华 王利民 欧阳仁荣 LIU Jia,CHEN Fang-yuan,WANG Hai-rong,et al.(1.Department of Hematology,2.Central laboratory,RenJi Hospital,Shanghai JiaoTong University School of Medicine,Shanghai 200001,China)
出处 《中国实验诊断学》 北大核心 2011年第4期 571-575,共5页 Chinese Journal of Laboratory Diagnosis
基金 国家自然科学基金资助项目(30670881)
关键词 磷酸氯喹 白血病细胞株 PNAS-2 凋亡 chloroquine phosphate leukemia cell lines PNAS-2 apoptosis
作者简介 刘佳,女,31岁,硕士,住院医师,研究方向:白血病的发病机制。 通讯作者
  • 相关文献

参考文献15

  • 1王海嵘,韩洁英,钟济华,顾春红,王婷,朱坚轶,陈芳源,欧阳仁荣.应用cDNA末端快速扩增法扩增PNAS-2基因5′端未知序列的实验研究[J].中华血液学杂志,2006,27(1):55-57. 被引量:5
  • 2Von Schwedler UK,Stuchell M,Müller B,et al.The protein network of HIV budding[J].Cell,2003,114(6):701. 被引量:1
  • 3Howard TL,Stauffer DR,Degnin CR,et al.CHMP1 functions as a member of a newly defined family of vesicle trafficking proteins[J].J Cell Sci,2001,114:2395. 被引量:1
  • 4Ward DM,Vaughn MB,Shiflett SL,et al.The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells[J].J Biol Chem,2005,280(11):10548. 被引量:1
  • 5Wang HR,Chen FY,Zhong H,et al.PNAS-2's subcullar location in leukemic cell is abnormal and its relation with leukemogenesis[J].Blood (ASH Annual Meeting Abstracts),2007,110:4299. 被引量:1
  • 6Wang HR,Gu CH,Zhu JY,et al.PNAS-2:A Novel Gene Probably Participating in Leukemogenesis[J].Oncology,2006,71(5-6):423. 被引量:1
  • 7王海嵘,顾春红,钟璐,钟济华,韩洁英,陈芳源,欧阳仁荣.抑制凋亡相关基因PNAS-2表达后U937细胞凋亡率的改变[J].诊断学理论与实践,2007,6(5):422-426. 被引量:4
  • 8Koranda FC.Antimalarials[J].J Am Acad Dermatol,1981,4(6):650. 被引量:1
  • 9Liu J,Chen FY,Wang HR,et al.Preparation and Identification of Monoclonal Antibody against PNAS-2 Protein[J].Zhongguo Shi Yan Xue Ye Xue Za Zhi,2009,17(5):1269. 被引量:1
  • 10Kranz A,Kinner A,K(o)lling R.A Family of small coiled-coil-forming Proteins Fuctioning at the Late Endosome in Yeast[J].Mol Bid Cell,2001,12(3):711. 被引量:1

二级参考文献6

共引文献5

同被引文献15

  • 1Xu SH, Xu H. Phytotoxins in juglans and their utilization[J]. J Shenyang Agri Univ, 1990, 21 (2): 167- 170. 被引量:1
  • 2Zhang YP, Yang ZB. The antiseptic effect of juglone and its inhibition effect on tumor cells multiplication [J]. J Shenyang PharmaceutUniv, 1993, 10 (4): 271- 274. 被引量:1
  • 3Lu KP, Finn G, Lee TH, et al. Prolylcis-trans isomerization as a molecular timer [J]. Nat Chem Biol, 2007, 3 (10).. 619-629. 被引量:1
  • 4Yi L, Zeng X, Su Q, et al. Establisment of the model of apoposis initiation phase in human lukemia HL-60 cells induced by DADS [ J ]. Chin Pharmacol Bull, 2007, 23 (9): 1250-1253. 被引量:1
  • 5Michelle TP, Mats L. The natural toxin juglone causesdegradation of p53 and induces rapid H2AX phosphorylation and cell death in human fibroblasts [J].Toxicol Appl Pharmacol, 2005, 209 (4):1-9. 被引量:1
  • 6Lillig CH, Holmgren A. Thioredoxin and related molecules from biology to health and disease[J]. Antioxid Redox Signal, 2007, 9 (1): 25- 47. 被引量:1
  • 7Gromer S, Urig S, Becker K. The thioredoxin system-from science to clinic[J].Med Res Rev, 2004, 24 (1): 40-89. 被引量:1
  • 8Nordberg J, Arn6r ESJ. Reactive oxygen species, antioxidants, and the mammalian thioredoxin system [J].FreeRadicBiolMed, 2001, 31 (11): 1287-1312. 被引量:1
  • 9RundlOf AK, Arn4r ESJ. Regulation of the mammalian selenoprotein thioredoxin reductase 1 in relation to cellular phenotype, growth and signaling events[J].Antiox Redox Signal, 2004, 6 (1):41 -52. 被引量:1
  • 10Chiu LC, Ho TS, Wong EY, et al. EthyIacetate extract of Patriniascabiosaefolia downregulates anti apoptotic Bcl-2/Bel XL expression, and induces apoptosis in human breast carcinoma MCF-2 cells independent of caspase-9 activation [J]. J Etbmopharmacol, 2006, 105 (1/2):263- 268. 被引量:1

引证文献1

二级引证文献8

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈