目的 探讨马鞍草醇提液（ａｅＶｏ）对绒毛膜癌ＪＡＲ细胞株的影响。方法 ＪＡＲ细胞株与人肝癌ＳＭＭＣ－７７２１细胞株及人胚胎２倍体成纤维细胞株（２ＢＳ）体外培养比较，应用溴化甲噻唑基四唑（ＭＴＴ）法、荧光法测得细胞相对生长曲线及增殖抑制率。结果 ａｅＶｏ对ＪＡＲ细胞有抑制作用，于加药后４８ｈ，增殖抑制率为（７１．９±４．０）％及（６９．６±２．）％，ＭＴＴ法及荧光法所测结果无明显差异（Ｐ〉０．０５）
Objective To study the effect of aeVo on choriocarcinoma JAR cells. Methods JAR cells, SMMC 7721 cells and 2BS cells were cultured in vitro and proliferation of these cells were detected using MTT method and fluorescent method. Results We observed that the proliferation of JAR cells were inhibited. 48 h after the addition of 25 g/L aeVo. The proliferation inhibition ratio determined by these two method, were (71.9±4.0)% and (69 6±2.0)% respectively. There was no significant difference between MTT method and fluorescent method ( P>0 05 ). AeVo exerted no obvious effects on SMMC 7721 cells and 2BS cells. Conclusion AeVo could inhibit JAR cell growth.
Acta Universitatis Medicinalis Nanjing
alcohol extract of Verbena officinalis
choriocarcinoma JAR cell