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鸭白细胞抗菌肽对小鼠免疫功能的影响 预览 被引量:5

Effects of Antimicrobial Peptides from Duck Leukocytes on Immunity of Mice
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摘要 从樱桃谷鸭血液中提取出白细胞并制备粗提抗菌肽,分别以低、中、高3种剂量(质量浓度分别为1.5 g/L、7.5 g/L、15 g/L)灌胃清洁级昆明系小鼠,0.2 mL/(d.只),同时设0.01%醋酸溶液对照组和蛋白对照组(10 g/L牛血清白蛋白)。连续灌胃14 d后,测定小鼠的免疫器官指数、E玫瑰花环形成率、碳粒廓清指数;同时采用流式细胞术测定小鼠外周血T细胞亚群的水平。结果表明,各剂量的白细胞抗菌肽均能显著提高小鼠的E玫瑰花环形成率和CD3+水平;同时15 g/L的白细胞抗菌肽能明显提高小鼠的免疫器官指数、巨噬细胞吞噬指数、CD4+、CD4+/CD8+水平。初步说明鸭白细胞粗提抗菌肽能明显增强小鼠的免疫功能。 Crude extractions of antimicrobial peptides from Yingtaogu duck blood was administrated in Kunming strain mice for two weeks through gavage at a daily dose of 0.2mL with the concentrations of 1.5g/L,7.5g/L or 15g/L,respectively.For negative controls,0.2mL of 0.01% acetic acid or 10g/L BSA was separately used.The serum phagocytic rate,phagocytic index,and weight of the immune organs(spleen and thymus) were measured,whilst the total number and subgroups of tlymphocytes were counted through flow cytometry(FCM).The results showed that the extract improved the E-rosette ratio and the total number of CD3+ T cells.Compared to other groups,the extract used at a dosage of 15mg/L significantly improved the immune organ weight index(P0.05),serum phagocytic index(P0.01),and the percentages of both CD4+ and CD8+ T cells(P0.01).This indicates that the immunity of mice was significantly improved by antimicrobial peptides extracted from duck leukocytes.
作者 耿娟 王永才 陈丽颖 GENG Juan,WANG Yong-cai,CHEN Li-ying (College of Animal Husbandry and Veterinary Sciences,Henan Agricultural University,Zhengzhou 450002,China)
出处 《河南农业科学》 CSCD 北大核心 2011年第4期 142-145,共4页 Journal of Henan Agricultural Sciences
基金 河南省科技攻关项目(0523010500) 河南省重点科技攻关项目(072102130020)
关键词 白细胞抗菌肽 免疫 流式细胞术 Duck Antimicrobial peptides from leukocytes Immunity Flow cytometry
作者简介 耿娟(1979-),女,河南新乡人,助理实验师,硕士,主要从事动物生长发育调控方面的研究。E-mail:genNuanzz@126.com 通讯作者:陈丽颖(1967-),女,河南南阳人,副教授,博士,硕士生导师,主要从事动物微生物与免疫学研究。E—mail:chenliying666@sina.com
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  • 1Donovan K I., Topley N. What are renal defensins de- fending? [J]. Nephron Exphrol, 2003,93 : 125-128. 被引量:1
  • 2潘行正,黄正明,李永新.抗菌肽制剂对母猪死产率和仔猪成活率的影响[J].现代农业科技,2010(12):285-286. 被引量:20
  • 3王万云,马志娟,周业飞.鸡小肠抗菌肽的提取及对肉鸡生产性能的影响[J].山西农业科学,2010,38(7):112-114. 被引量:7
  • 4Brockus C W,Jackwooct M W, Harman B G. Characte rization of beta-defensin prepropeptide mRNA from chicken and turkey bone marrow[J]. Anita Genet, 1998,29:283 289. 被引量:1
  • 5赵飞.家蝇抗菌活性物质研究进展[J].山西农业科学,2007(9):23-26. 被引量:13
  • 6Yang D,Biragyn A,Kwak I.W, et al. Mammalian defensins in immunity: more than just microbieidal [J]. Trends Immunol, 2002,23 (6) : 291-296. 被引量:1
  • 7Stolzenberg E D,Anderson G M, Ackermann M R, et al. Epithelial antibiotic induced in states of disease[J]. Proc Natl Acad Sci USA,1997,94(16)..8686-8690. 被引量:1
  • 8Zhang G, Ross C R, Dritz S S, et al. Salmonella infection increases porcine antibacterial peptide concentrations in serum[J]. Clin Diagn Lab Immunol, 1997, 4 (6) : 774- 777. 被引量:1
  • 9O'Neil D A,Porter E M,Elewaut D,et al. Expression and regulation of the human beta-defensins hBD- 1 and hBD-2 in intestinal epithelium[J]. J Immunol, 1999, 163(12) .-6718-6724. 被引量:1
  • 10Wilson C L,Ouellette A J, Satchell D P,et al. Regu- lation of intestinal alpha-defensin activation by the metalloproteinase matrilysin in innate host defense[J]. Science, 1999,286:113. 被引量:1

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