期刊文献+

东北民猪荷包猪抗性基因多态性及其与免疫指标的相关性研究

Relationship between polymorphisms of the genes associated with disease resistances and immune traits in the Northeast Hebao Pigs
收藏 分享 导出
摘要 研究目的是检测荷包猪FUT1和Mxl基因位点多态性及其与免疫指标的相关关系。采用PCR—RFLP技术分析基因多态性,采用ELISA方法检测免疫指标白介素4(IL-4)、分泌型免疫球蛋白A(sIgA)、α-干扰素(IFN-α)的表达量。结果表明,荷包猪FUT1基因和Mxl基因Hin6I点酶切位点上显示多态性,优势基因型分别为GG和AA型,在Mxl基因其他两位点处未发现多态性;荷包猪免疫指标IL-4、srgA、IFN—α的表达量明显高于大白猪(P〈0.05),但抗性基因型与免疫指标间的相关性表现不明显。 In order to analyze the polymorphism of FUT 1 and Mxl genes of Hebao Pigs, and the correlativity between polymorphism and immune traits, the PCR-RFLP was used to analyse the polymorphism of genes and the double-antibody sandwich enzyme-linked immunoassay (ELISA) was used to analyse the level of interferon-or (IFN-c0, interleukin-4 (IL-4) and secretory immunoglobulin A (sIgA). Results indicated that Hebao Pigs had polymorphism with Hin6I of FUT 1 and Mxl genes and the advantages of genotypes were GG and AA, other sites didn't have polymorphism; and the levels of IFN-α, IL-4 and SIgA in Hebao pigs were higher those in Large White pigs. Our results suggested that the relativity between polymorphisms of genes associated with disease resistance and immune traits was not noticeable.
作者 朱弘焱 赵颂 巴彩凤 苏玉虹 ZHU Hong-yan, ZHAO Song, BA Cai-feng , SU Yu-hong (1College of Animal Husbandry and Veterinary Medicine, Liaoning Medical University, Jinzhou 121000, China; 2. Experimental Center of Science, Liaoning Medical University, Jinzhou 121000, China; 3. Experimental Animal Center, Liaoning Medical University, Jinzhou 121000, China)
出处 《畜牧与兽医》 北大核心 2011年第8期11-16,共6页 Animal Husbandry & Veterinary Medicine
基金 辽宁省科技厅科技项目(编号:2009408-6)
关键词 荷包猪 FUT1 Mx1 PCR-RFLP 白介素-4 分泌型免疫球蛋白A Α-干扰素 Hebao pigs FUT1 gene Mxl gene PCR-RFLP IL-d sIgA IFN-α
作者简介 朱弘焱(1984-),女,助理实验师,硕士,研究方向:动物分子遗传学。 通讯作者:苏玉虹,女,教授,博士,研究方向:动物分子遗传学。
  • 相关文献

参考文献10

二级参考文献61

共引文献53

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈