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小麦赤霉病流行区镰刀菌致病种及毒素化学型分析 被引量:23

Analysis on the population structure of Fusarium pathogenic spp.and its mycotoxin chemotypes in Fusarium head blight epidemic region
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摘要 为从分子水平上明确小麦赤霉病流行区镰刀菌致病种及其B型毒素化学型的分布特点,本研究对2008年度采自四川、重庆、湖北、安徽、江苏、河南6省33县市的赤霉病穗上分离获得的433个镰刀菌单孢菌株,用鉴定种和鉴定B型毒素化学型的特异性引物进行了鉴定分析。致病种检测结果表明,四川病穗检测到Fusarium asiaticum、F.graminearum、F.avenaceum和F.meridionale 4个镰刀菌种,重庆、湖北、安徽和江苏病穗检测到F.asiaticum和F.graminearum 2个种,河南病穗仅检测到F.graminearum 1个种。毒素化学型检测结果表明,Nivalenol(NIV)是四川和重庆镰刀菌主要毒素化学型,Deoxynivalenol(DON)是湖北、河南、安徽和江苏镰刀菌主要毒素化学型;将DON化学型进一步划分为3-AcDON和15-AcDON显示,四川、湖北、江苏镰刀菌毒素以3-AcDON为主,安徽镰刀菌毒素为3-AcDON和15-AcDON两者参半,河南镰刀菌全部产生15-AcDON。结果揭示,F.asiaticum是四川、重庆、湖北和江苏等赤霉病流行麦区的优势致病种;镰刀菌产生的DON和NIV毒素化学型存在明显的地域分布,长江上游的麦区以NIV为优势化学型,长江中下游麦区以DON为优势化学型;镰刀菌致病种与DON毒素的化学型间存在一定关系。 A broad survey was conducted and infected heads of wheat were collected from 33 counties in Sichuan,Chongqin,Hubei,Anhui,Jiangsu and Henan Provinces in 2008.Totally 433 Fusarium single spore isolates were obtained and the population and mycotoxin chemotypes had been studied through PCR assay.Four Fusarium species,F.asiaticum,F.graminearum,F.avenaceum and F.meridionale,were detected in Sichuan,while two species F.asiaticum and F.graminearum were detected in Chongqing,Hubei,Anhui and Jiangsu.In Henan Province,only F.graminearum was found.Chemotype detection results showed that Nivalenol was the main chemotype in Sichuan and Chongqing,while Deoxynivalenol was the major chemotype in Hubei,Henan,Anhui and Jiangsu.After further divided the DON chemotype strains into 3-AcDON and 15-AcDON,the results showed that 3-AcDON isolates mainly lied in Sichuan,Hubei and Jiangsu,both 3-AcDON and 15-AcDON isolates mixed in Anhui,and all the isolates in Henan belonged to 15-AcDON.The results indicated that F.asiaticum was the dominant specie in Sichan,Chongqing,Hubei and Jiangsu Province.It also showed that there was a clear geographical distribution of DON and NIV produced by Fusarium species.NIV was the dominant chemotype in wheat producing region belonged to the upper reaches of Yangtze River while DON was the dominant chemotype in the lower reaches.There were some relationship between DON chemotype and Fusarium species.
作者 史文琦 杨立军 冯洁 张旭 曾凡松 向礼波 汪华 喻大昭 SHI Wen-qi,YANG Li-jun,FENG Jie,ZHANG Xu,ZENG Fan-song,XIANG Li-bo,WANG Hua,YU Da-zhao(1Institute for Plant Protection and Soil Sciences,Hubei Academy of Agricultural Sciences;Hubei Key Laboratory of Crop Diseases,Insect Pests and Weeds Control,Wuhan 430064,China;2Institute of Plant Protection,Chinese Academy of Agricultural Scieces;State Key Laboratory for Biology of Plant Disease and Insect Pests,Beijing 100193,China;3Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处 《植物病理学报》 CAS CSCD 北大核心 2011年第5期 486-494,共9页 Acta Phytopathologica Sinica
基金 国家“十一五”支撑计划(2006BAD08A05) 农业部公益性行业科研专项(3-15)
关键词 小麦 赤霉病 镰刀菌种群结构 毒素化学型 wheat Fusarium head blight population structure mycotoxin chemotype
作者简介 史文琦(1983-),女,江苏盐城人,研究实习员,硕士,主要从事小麦真菌病害检测及其分子生物学研究;Tel:134371315668&027-87380681,E-mail:iamjiagjing.hi@163.com。 通讯作者:喻大昭,研究员,主要从事小麦病害和植物源杀菌剂研究;E-mail:Dazhaoyu@china.com
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