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草莓果实中脱落酸受体基因FaABAR/CHLH表达变化及其影响因素分析 被引量:9

Expression Changes of ABA Receptor Gene FaABAR/CHLH in Strawberry and Its Affection Factors
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摘要 为了探讨脱落酸调控草莓果实成熟的信号识别机制,以‘北农香’草莓花后1~4周的果实为试材,在克隆脱落酸受体基因FaABAR/CHLH的基础上,通过荧光定量PCR研究了果实发育过程中该基因表达量的变化及其影响因素。结果表明,草莓果实中脱落酸受体FaABAR/CHLH基因编码一个含有1381个氨基酸的蛋白,且该蛋白含有一个金属离子螯合酶H亚基超家族保守区。在果实发育的前期(小绿、大绿和浅绿果期),FaABAR/CHLH基因表达量维持较高水平;随着果实的加速褪绿,其表达量迅速降低,至白果期降到最低点;随着果实着色启动,其表达量又迅速上升。ABA和高pH能促进FaABAR/CHLH基因在转录水平上的表达,而蔗糖则抑制该基因转录水平。草莓果实中脱落酸受体基因FaABAR/CHLH表达水平的变化进一步揭示了ABA在调控果实成熟中的重要作用。 To investigate the signalling perception mechanism of ABA regulation on strawberry fruit ripening,experiment with'Beinongxiang'strawberry fruit during 1-4 weeks after anthesis,the mRNA expression level of FaABAR/CHLH gene in developing fruit and its affection factors were detected via real-time PCR and fruit-tissue discs.The results showed that a 4 325 bp cDNA fragment encoded a strawberry ABA receptor FaABAR/CHLH protein with 1 381 amino acid sequences that contain a conserved domain of metal ion chelatase H subunit superfamily.During the early developmental stage of frui(tsmall green,large green,and light green),the FaABAR/CHLH gene expressed highly.Coupled with fruit de-greening,the transcription level of the gene rapidly decreased,and then up to the lowest level at white stage.On the beginning of fruit red-coloring,the transcription level of the gene rapidly increased.Both ABA and pH could markedly promote the mRNA expression level of the gene.In contrast,sucrosesignificantly inhibited the gene transcripts.The change of the mRNA expression level of ABA receptor gene FaABA/CHLH during strawberry fruit development further uncovered an important role of ABA in regulation of fruit ripening.
作者 贾海锋 柴叶茂 李春丽 董清华 秦岭 沈元月 JIA Hai-feng,CHAI Ye-mao,LI Chun-li,DONG Qing-hua,QIN Ling,and SHEN Yuan-yue(Beijing Key Laboratory for Agricultural Application and New Technique,College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China)
出处 《园艺学报》 CAS CSCD 北大核心 2011年第9期1650-1656,共7页 Acta Horticulturae Sinica
基金 国家自然科学基金项目(30971977) 北京市自然科学基金项目(6082005) 北京市自然科学基金和北京市教委联合资助重点项目(KZ200910020001)
关键词 草莓 果实 脱落酸受体ABAR/CHLH 基因 表达 蔗糖 PH strawberry fruit abscisic acid receptor ABAR/CHLH gene expression sucrose pH
作者简介 通信作者 (E-mail:sfmn@tom.com)
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