期刊文献+

NTIS大鼠血清及脑组织甲状腺激素水平及其转运蛋白OATP1C1和MCT8 mRNA变化 被引量:1

Changes of thyroid hormone and mRNA of OATP1C1 and MCT8 in serum and brain of rat with nonthyroidal illness syndrome
收藏 分享 导出
摘要 目的观察非甲状腺疾病综合征(nonthyroidal illness syndrome,NTIS)大鼠血清及脑组织甲状腺激素水平及其转运蛋白有机阴离子转运多肽(organic anion transporting polypeptide 1C1,OATP1C1)和单羧酸转运蛋白(monocarboxylatetransporter 8,MCT8)mRNA的变化。方法选取成年SPF级SD雄性大鼠23只,分为正常组(n=12)和NTIS组(n=11)。NTIS动物采用肝纤维化模型,利用四氯化碳(1∶6)用玉米油稀释,0.025 ml腹腔注射,3次/周,共8周。正常组大鼠相同剂量玉米油,腹腔注射,3次/周,共8周。造模成功后2组利用水迷宫法测试学习记忆能力。随后处死,取血及脑组织,放免法测定血清甲状腺激素(T3、T4、rT3、TSH)及脑组织甲状腺激素(T3、T4、rT3);RT-PCR法测定脑组织中OATP1C1及MCT8 mRNA水平。结果①NTIS组血清T3、T4水平较正常组明显降低[T3(0.53±0.18)mmol/L vs(0.73±0.22)mmol/L,P〈0.05;T4(24.58±7.73)mmol/L vs(39.97±14.74)mmol/L,P〈0.01],rT3、TSH水平2组之间无显著性差异(P〉0.05);NTIS组脑组织中T3、T4浓度较正常组稍升高,但差异无统计学意义(P〉0.05);②NTIS组OATP1C1和MCT8 mRNA水平较正常组明显升高[OATP1C1 mRNA(0.054±0.032)vs(0.019±0.016),P〈0.01;MCT8 mRNA(0.058±0.048)vs(0.017±0.012),P〈0.05];③2组大鼠空间探索和定位航行之间无显著性差异(P〉0.05)。结论 NTIS大鼠脑内通过OATP1C1和MCT8代偿,保持脑内T3、T4水平相对稳定,从而维持中枢甲状腺功能正常。 Objective To investigate the changes of thyroid hormone level and mRNA expressions of thyroid hormone transporter organic anion transporting polypeptide1C1(OATP1C1) and monocarboxylate transporter 8(MCT8) in the sera and brain tissues of rats with nonthyroidal illness syndrome(NTIS).Methods Twenty-three male SD rats were divided into a control group(n=12) and a NTIS group(n=11).The rats of the NTIS group and the control group were given intraperitoneal injection of carbon tetrachloride(CCl4)(1∶6,diluted by corn oil) 0.025 ml and corn oil 0.025 ml,respectively,3 times/week for 8 weeks.Morris water-maze test was used to detect the ability of learning and memory of the rats.Then the rats were sacrificed and sera and brain tissues were collected.The levels of thyroid hormone(TH) in the sera(T3,T4,rT3 and TSH) and brain tissues(T3,T4 and rT3) were detected by radioimmunoassay(RIA),and the mRNA levels of OATP1C1 and MCT8 in the brain tissues were detected by RT-PCR.Results(1) The levels of T3 and T4 in the sera of the NTIS group decreased as compared with those of the control group [T3(0.53±0.18) mmol/L vs(0.73±0.22) mmol/L,T4(24.58±7.73) mmol/L vs(39.97±14.74) mmol/L;P0.05,P0.01].The levels of T3 and T4 in the brain tissues showed no significant difference between the two groups,although they slightly increased in the NTIS group.The levels of TSH and rT3 in the sera and brain tissues showed no significant differences between the 2 groups(P0.05).(2) The mRNA expressions of OATP1C1 and MCT8 in the brain tissues increased significantly in the NTIS group as compared with those in the control group.(3) The abilities of space exploration and place navigation of the rats between the 2 groups showed no significant difference(P0.05).Conclusion The compensated upregulations of OATP1C1 and MCT8 contribute to maintain a relatively stable TH level in the brains of rats with NTIS,so as to maintain normal functions of thyroid.
作者 袁大华 王群 Yuan Dahua,Wang Qun(1Department of Neurology,First People's Hospital of Foshan,Foshan,Guangdong Province,528000;2Department of Neurology,Nanfang Hospital,Southern Medical University,Guangzhou,Guangdong Province,510515,China)
出处 《第三军医大学学报》 CAS CSCD 北大核心 2012年第6期 517-521,共5页 Acta Academiae Medicinae Militaris Tertiae
关键词 非甲状腺疾病综合征 甲状腺激素 认知 转运蛋白 nonthyroidal illness syndrome thyroid hormone cognition thyroid hormone transporter
作者简介 [通信作者]王群,电话:(020)62741965,E—mail:zhpn@fimmu.com
  • 相关文献

参考文献23

  • 1Yen P M.Physiological and molecular basis of thyroid hormone action[J].Physiol Rev,2001,81(3):1097-1142. 被引量:1
  • 2Visser W E,Friesema E C,Visser T J.Minireview:thyroid hormonetransporters:the knowns and the unknowns[J].Mol Endocrinol,2011,25(1):1-14. 被引量:1
  • 3Laurberg P.Thyroid function:thyroid hormones,iodine and the brain-animportant concern[J].Nat Rev Endocrinol,2009,5(9):475-476. 被引量:1
  • 4Davis J D,Stern R A,Flashman L A.Cognitive and neuropsychiatricaspects of subclinical hypothyroidism:significance in the elderly[J].Curr Psychiatry Rep,2003,5(5):384-390. 被引量:1
  • 5李永军,王群.初次搜索策略对大鼠Morris水迷宫试验成绩的影响[J].中国现代医学杂志,2004,14(21):7-10. 被引量:5
  • 6Escobar-Morreale H F,Obregon M J,Escobar-del-Rey F,et al.Tissue-specific patterns of changes in 3,5,3'-triiodo-L-thyronineconcentrations in thyroidectomized rats infused with increasing doses ofthe hormone.Which are the regulatory mechanisms?[J].Biochimie,1999,81(5):453-462. 被引量:1
  • 7Gerges N Z,Alzoubi K H,Park C R,et al.Adverse effect of the com-bination of hypothyroidism and chronic psychosocial stress on hippo-campus-dependent memory in rats[J].Behav Brain Res,2004,155(1):77-84. 被引量:1
  • 8Economidou F,Douka E,Tzanela M,et al.Thyroid function duringcritical illness[J].Hormones(Athens),2011,10(2):117-124. 被引量:1
  • 9Adler S M,Wartofsky L.The nonthyroidal illness syndrome[J].Endocrinol Metab Clin North Am,2007,36(3):657-672,vi. 被引量:1
  • 10De-Groot L J.Dangerous dogmas in medicine:the nonthyroidal illnesssyndrome[J].J Clin Endocrinol Metab,1999,84(1):151-164. 被引量:1

二级参考文献10

  • 1刘俊艳,赵淑蓉.急性脑血管病患者下丘脑—垂体—甲状腺轴功能...[J].中华神经精神科杂志,1993,26(1):31-34. 被引量:44
  • 2陈家伦.内科疾病中血甲状腺素和淋巴细胞T3受体的变化[J].中华内科杂志,1987,26(5):501-501. 被引量:2
  • 3侯熙德.神经病学:第3版[M].北京:人民卫生出版社,1990.108-118. 被引量:1
  • 4王颖慧 张茂林 段建平 等.脑梗死患者血清T3、T4、TSH测定及临床意义[J].新医学,1994,26(5):247-248. 被引量:1
  • 5Morris R. Developments of a water-maze procedure for studying spatial learning in the rat[J]. J Neurosci Methods. 1984, 11: 47-60. 被引量:1
  • 6Robert N, Scott M, Richard E et al. Tests used to assess the cognitive abilities of aged rats: Their relation to each other and to hippocampus morphology and neurotrophin expression[J]. Gerontology,1999, 45(3): 143-152 被引量:1
  • 7Wickelgren, Ingrid. Is hippocampal cell death a myth[J]? Science,1996, 271(5253): 1229-1230 被引量:1
  • 8I-Ching Chou, Tatyana Trakht, Carina Signori, et al. Behavioral/environmental intervention improves learning after cerebral hypoxia-ischemia in rats[J]. Stroke, 2001, 32(9): 2192-2197 被引量:1
  • 9Philip L, Rhonda L, William E. Chronic [D-Ala(2)]-growth hormone-releasing hormone administration attenuates age-related deficits in spatial memory. The Journals of Gerontology: Series A:Biological sciences and medical sciences, 2000, 55(2): B106-113 被引量:1
  • 10P E Wainwright, H -C Xing, G R Ward, et al. Water maze performance is unaffected in artificially reared rars fed diets supplemented with arachidonic acid and docosahexaenoic acid[J]. The Journal of Nutrition, 1999, 129(5): 1079-1088. 被引量:1

共引文献9

同被引文献20

  • 1杨志强,王锐,任剑羽.甲状腺激素与老年轻度认知障碍患者认知功能的相关性[J].中国老年学杂志,2014(8):2046-2047. 被引量:7
  • 2Berent D, Zboralski K, Orzechowska A, et al. Thyroid hor- mones association with depression severity and clinical out- come in patients with major depressive disorder[J]. Mol Biol Rep,2014,41(4) :2 419-2 425. 被引量:1
  • 3Mann JJ, McBride PA, Malone KM, et al. Blunted seroton- ergie responsivity in depressed inpatients[J]. Neuropsycho- pharmacology, 1995,13(1) : 53- 64. 被引量:1
  • 4Kupka RW, Nolen WA, Post RM, et al. High rate of auto- immune thyroiditis in bipolar disorder: lack of association with lithium exposure[J]. Biol Psychiatry, 2002,51 : 305- 331. 被引量:1
  • 5Homan P, Drevets WC, Hasler G. Neural correlates of free T3 alteration after catecholamine depletion in subjects with re- mitted major depressive disorder and in controls[J]. Psycho- pharmacology (Berl) ,2014, 231(2) :409-417. 被引量:1
  • 6Kobayashi T, Kato S. Depression- dementia medius: Be- tween depression and the manifestation of dementia symptoms [J]. Psychogeriatrics,2011,11(3) :177-182. 被引量:1
  • 7Aoun EG, Lee MR, Haass-Koffler CL, et al. Relationship between the thyroid axis and alcohol craving[J]. Alcohol Al- cohol,2015,50(1) :24-29. 被引量:1
  • 8Volpato S, Guralnik J, Fried L, et al. Serum thyroxine level and cognitive decline in euthyroid older women[J]. Neurolo- gy,2002,58(7) :1 055-1 061. 被引量:1
  • 9Wahlin A, Bunce D, Wahlin TB. Longitudinal evidence of the impact of normal thyroid stimulating hormone variations on cognitive functioning in very old age[J]. Psychoneuroendocri- nology, 2005,30(7) : 625 - 637. 被引量:1
  • 10Kalmijn S, Mehta KM, Pols HA, et al. Subclinical hyperthy- roidism and the risk of dementia:the Rotterdam Study [J]. Clin Endoerinol, 2010,53 (6) : 733- 737. 被引量:1

引证文献1

二级引证文献6

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈