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Uric acid promotes neuronal differentiation of human placenta-derived mesenchymal stem cells in a time- and concentration-dependent manner 预览

Uric acid promotes neuronal differentiation of human placenta-derived mesenchymal stem cells in a time- and concentration-dependent manner
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摘要 Uric acid is an important, naturally occurring serum antioxidant. The present study investigates the use of uric acid for promoting proliferation and neuronal differentiation of mesenchymal stem cells derived from human placenta tissue. Human placenta-derived mesenchymal stem cells were pre-induced in the presence of either 0, 0.2, 0.4 or 0.8 mM uric acid in combination with 1 mM β-mercaptoethanol for 24 hours, followed by exposure to identical uric acid concentrations and 5 mM β-mercaptoethanol for 6 and 10 hours. Cells developed a neuronal-like morphology, with formation of interconnected process extensions, typical of neural cells. Immunocytochemistry and immunofluorescence staining showed neuron specific enolase positive cells were present in each group except the control group. A greater number of neuron specific enolase positive cells were observed in 0.8 mM uric acid in combination with 5 mM β-mercaptoethanol at 10 hours. After 24 hours of induction, Nissl bodies were detected in the cytoplasm of all differentiated cell groups except the control group and Nissl body numbers were greatest in human placenta-derived mesenchymal stem cells grown in the presence of 0.8 mM uric acid and 5 mM β-mercaptoethanol. These results suggest uric acid accelerates differentiation of human placenta-derived mesenchymal stem cells into neuronal-like cells in a time-and concentration-dependent manner. Uric acid is an important, naturally occurring serum antioxidant. The present study investigates the use of uric acid for promoting proliferation and neuronal differentiation of mesenchymal stem cells derived from human placenta tissue. Human placenta-derived mesenchymal stem cells were pre-induced in the presence of either 0, 0.2, 0.4 or 0.8 mM uric acid in combination with 1 mM β-mercaptoethanol for 24 hours, followed by exposure to identical uric acid concentrations and 5 mM β-mercaptoethanol for 6 and 10 hours. Cells developed a neuronal-like morphology, with formation of interconnected process extensions, typical of neural cells. Immunocytochemistry and immunofluorescence staining showed neuron specific enolase positive cells were present in each group except the control group. A greater number of neuron specific enolase positive cells were observed in 0.8 mM uric acid in combination with 5 mM β-mercaptoethanol at 10 hours. After 24 hours of induction, Nissl bodies were detected in the cytoplasm of all differentiated cell groups except the control group and Nissl body numbers were greatest in human placenta-derived mesenchymal stem cells grown in the presence of 0.8 mM uric acid and 5 mM β-mercaptoethanol. These results suggest uric acid accelerates differentiation of human placenta-derived mesenchymal stem cells into neuronal-like cells in a time-and concentration-dependent manner.
作者 Nailong Yang Lili Xu Peng Lin Jing Cui Yang, Nailong[2];Xu, Lili[2];Lin, Peng[3];Cui, Jing[1];
出处 《中国神经再生研究:英文版》 SCIE CAS CSCD 2012年第10期 756-760,共5页 Neural Regeneration Research
基金 Acknowledgments: We thank the Department of Obstetrics and Gynecology of Qingdao Marie Hospital in China for the placenta tissues and the Gout Laboratory of the Affiliated Hospital of Qingdiao University Medical College for their cell culture technical assistance and use of experimental equipment.
关键词 间充质干细胞 浓度依赖性 神经分化 人胎盘 神经元特异性烯醇化酶 尿酸 时间 巯基乙醇 uric acid human placenta-derived mesenchymal stem cells differentiation neural cells
作者简介 Nallong Yang, Master, Chief physician, Department of Endocrinology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China Corresponding author: Jing Cui, Studying for master's degree, VIP Department, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China cuijing512 @ 163.com
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