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草莓果实β-葡萄糖苷酶基因FaBG1克隆及成熟期表达分析 预览 被引量:1

Cloning and transcription analysis of β-glucosidase gene FaBG1 during strawberry fruit ripening
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摘要 为了探讨脱落酸合成相关酶β-葡萄糖苷酶FaBGl基因是否参与草莓果实成熟的调控,本研究以‘红颜’草莓着色期间的果实为试材,首先通过RT-PCR技术克隆β-葡萄糖苷酶FaBGl基因的鳊码区核苷酸序列。该1,845bp基因在基因组中以单拷贝形式存在,并编码1个含有615氨基酸的多肽。生物信息学分析表明,FaBG7含有5个跨膜区,在进化上高度保守。围绕果实着色期间FaBGt基因转录水平以及ABA含量分析表明,随着果实的着色增加,FaBG7转录水平和ABA含量都逐渐增加,半红后基因表达量逐渐降低,ABA含量继续上升,至成熟时述到高峰。本研究结果表明FaBG7可能参与革摹果实的着色启动。 This study investigated whether β-glucosidase FaBG1 is involved in the regulation of strawberry fruit ripening. At first FaBG1 gene was cloned by RT-PCR technique with "Hongyan" red-coloring fruit as experiment material. The gene encodes a polypeptide containing 1, 381 amino acids, and harbors in genomic DNA with a single copy. Bioinformatics analysis showed that FaBG1 was evolutionarily conserved, and contained three putative transmembrane domains. Analysis of both transcription levels of FaBG1 and ABA contents during fruit red-coloring showed that coupled with fruit red-coloring, levels of both FaBG1 expression and ABA content increased gradually; after half of fruit red-coloring period, the former declined, in contrast, the latter continually increased and reached peak at fruit ripening. The results suggested the gene FaBG1 might participate in the onset of fruit red-coloring.
作者 董银行 郭家选 DONG Yin-hang, GUO Jia-xuan
出处 《中国园艺文摘》 2012年第5期1-4,163共5页
基金 北京市自然科学基金和北京市教委联合资助重点项目(KZ200910020001).
关键词 草莓 果实成熟 脱落酸 Β-葡萄糖苷酶 基因克隆 Strawberry Fruit ripening Abscisic acid β-glucosidase Gene clone
作者简介 董银行(1983-),男,硕士;主要研究方向为果实发育激素调控。 通讯作者:郭家选,教授。guojx_hh@163.com
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  • 1李莉,杨雷,杨莉,郝保春.草莓果实生长发育及主要营养成分变化规律研究[J].江西农业学报,2006,18(2):67-70. 被引量:17
  • 2REN HuiBo FAN YiJian GAO ZhiHui WEI KaiFa LI GuiFen LIU Jing CHEN Lin LI BingBing HU JianFang JIA WenSuo.Roles of a sustained activation of NCED3 and the synergistic regulation of ABA biosynthesis and catabolism in ABA signal production in Arabidopsis[J].中国科学通报:英文版,2007,52(4):484-491. 被引量:5
  • 3谢鸣,陈俊伟,秦巧平,蒋桂华,孙崇波,张慧琴,徐红霞.转化酶和己糖激酶调控草莓聚合果内糖积累[J].植物生理与分子生物学学报,2007,33(3):213-218. 被引量:14
  • 4吴少伯 黄海 等.植物激素脱落酸的放射免疫测定[J].植物生理学通讯,1985,(1):44-46. 被引量:6
  • 5周燮 徐义俊 等.脱落酸的放射免疫测定法(RIA)[J].南京农业大学学报,1985,(1):89-91. 被引量:1
  • 6Chernys J T, Zeevaart J A D. 2000. Characterrization of the 9-cis-epoxycaroteniod dioxygenase gene family and regulation of abscisic acid biosynthesis in avocado. Plant Physiol, 124:343 - 353. 被引量:1
  • 7Kato M, Matsumoto H, Ikoma Y. 2006. The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit. Journal of Experimental Botany, 57 (10): 2153 - 2164. 被引量:1
  • 8Lee K H, Piao H L, Kim H Y, Choi S M, Jiang F, Hartung W, Hwang I, Kwak J M, Lee I J, Hwang I. 2006. Activation of glucosidase via stress-induced polymerization rapidly increases active pools ofabscisic acid. Cell, 126: 1109 - 1120. 被引量:1
  • 9Lefebvre V, North H, Frey A, Sotta B, Seo M, Okamoto M, Nambara E, Annie M P. 2006. Functional analysis ofArabodopsis NCED6 and NCED9 genes indicate that ABA synthesized in the endosperm is involved in the induction of seed dormancy. Plant Physiol Biochem, 37:341 - 350. 被引量:1
  • 10Luchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kazuko Y S, Shinozaki K. 2001. Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis. Plant J, 27:325 - 333. 被引量:1

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