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铁剥夺和铁超负荷对白血病细胞株HL-60凋亡的影响机制 预览 被引量:2

The influence of iron deprivation and rich iron on the apoptosis of human Leukemia-60 cells
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摘要 目的 探讨铁剥夺和铰超负荷对白血病细胞株HL-60细胞凋亡的影响及其机制,为临床采用铁剥夺策略治疗或辅助治疗白血病提供理论依据.方法 在HL-60细胞培养基中分别加入不同浓度的去铁胺(DFO)或三氯化铁(FeCl3),造成细胞内铁剥夺或铁超负荷状态,采取噻唑蓝(MTT)法、DNA原位末端标记染色法(TUNEL)、免疫组化法检测铁剥夺和铁超负荷状态下HL-60细胞活力、凋亡率、细胞色素C(Cyt C)阳性细胞率.结果 DFO组细胞活力呈明显下降趋势,凋亡率呈显著上升趋势;FeCl3组细胞活力和凋亡率与对照组相比呈下降趋势;DFO组细胞胞浆内Cyt C阳性细胞率与对照组相比明显升高;而FeCl3组细胞浆内CytC阳性细胞率与对照组相比无明显差异.结论 铁剥夺可促进线粒体释放Cyt C,诱导HL-60细胞凋亡;铁超负荷对线粒体释放Cyt C无直接影响作用. Objective To explore the influence and mechanism of iron deprivation and rich iron on the apoptotic process of human Leukemia-60 (HL-60) cells, and provide the theoretical basis for the clinical therapy. Methods HL-60 ceils were cultivated with different concentration of DFO and FeCl3 to establish intracellular iron-deprivated and rich-iron states. Cell viability, apoptotic rate and the positive rate of Cyt C were detected by MTT, TUNEL and immunohistochemistry. Results The cell viability in DFO-treated group decreased, while the apoptotic rate increased dramatically. In contrast, an opposite result was obtained in FeCl3-treated group. The positive rate of Cyt C in DFO-treated group was elevated, but no difference was found in FeCl3-treated group. Conclusions Iron deprivation induces apoptosis of HL-60 cells by releasing Cyt C from mitochondria, while rich iron has no direct impact on it. The mechanism of HL-60 cell proliferation might be related with the apoptosis-related genes in the upstream of the mitoehondrial pathway.
作者 王叨 李四保 刘玉峰 WANG Dao, LI Sibao, LIU Yufeng. Department of Pediatrics, the First Affiliated Hospital of Zhengzhou University;2 Department of Pediatrics, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
出处 《中国小儿血液与肿瘤杂志》 CAS 2012年第3期110-112,共3页 Journal of China Pediatric Blood and Cancer
关键词 铁剥夺 铁超负荷 细胞凋亡 细胞色素C Iron deprivation Iron overload Human leukemia-60 cell Apoptosis Cytochrome C
作者简介 通讯作行:刘玉峰,Email:l-y6012@tom.com
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  • 1Celine C, Coulon S, Naudin J, et al. Targeting iron homeostasis induces cellular differentiation and synergizes with differentiating agents in acute myeloid leukemia. J Exp Med, 2010, 207: 731-750. 被引量:1
  • 2Noulsri E, Richardson DR, Lerdwana S, et al. Antitumor activity and mechanism of action of the iron chelator, Dp44mT, against leukemic cells. Am J Hematol, 2009, 84: 170-176. 被引量:1
  • 3Ganguly A, Basu S, Chakraborty P, et al. Targeting mitochondrial cell death pathway to overcome drug resistance with a newly developed iron chelate. PLoS One, 2010, 5 : el 1253. 被引量:1
  • 4Le NT, Richardson DR. The role of iron in cell cycle progression and the proliferation of neoplastic cells. Biochim Biophys Acta, 2002, 1603: 31-46. 被引量:1
  • 5Fu D, Richardson DR. Iron chelation and regulation of the cell cycle: 2 mechanisms of post- transcriptional regulation of the universal cyclin-dependent kinase inhibitor p21CIP1/WAF1 by iron depletion. Blood, 2007, 110: 752-761. 被引量:1
  • 6Kelvin Cain, Sha'tm B, Bratton, et al. The Apaf-I apoptosome: a large easpase-activating complex. Biochimie, 2002, 9 : 203-214. 被引量:1
  • 7I-lalestrap AP, Doran E, Gillespie JP, et al. Mitochondria and ceil death. Biochem Soc Trans, 2000, 28: 170-177. 被引量:1
  • 8张秋堂,王叨,刘玉峰.铁对白血病细胞HL-60线粒体膜电位及凋亡的影响[J].实用儿科临床杂志,2006,21(15):998-999. 被引量:5

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