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实验性肿瘤细胞转移动物模型的活体成像观察 预览 被引量:2

Dynamic Observation on In vivo Bioluminescence Imaging of Experimental Metastatic Animal Models in Nude Mice
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摘要 目的利用小动物活体成像系统观察肿瘤细胞在动物体内的转移情况。方法分别将不同浓度的绿色荧光蛋白(GPF)和荧光素酶(luciferase,Luc)双标的SMMC-7721细胞接种入裸小鼠尾静脉和脾,建立实验性转移动物模型,采用活体成像技术监测不同浓度的细胞在小鼠体内的转移情况,动态观察同一细胞于不同时间点在小鼠体内的转移情况。结果成功建立了尾静脉接种肺转移及脾内接种肝转移的实验性转移动物模型,经小动物活体成像系统检测发现,随着接种细胞浓度的增加,荧光素的表达面积和强度逐渐增加,二者呈正比关系;随着接种时间的延长,荧光素的表达面积和强度逐渐减弱,二者呈成反比关系。结论活体荧光成像系统可较好地观测肿瘤在动物体内深部脏器的转移情况,它将为肿瘤转移机制、抗转移治疗等研究提供有益的帮助。 Objective To observe the tumor metastasis in deep organisms of the nude mice by in vivo bioluminescence imaging system. Methods The SMMC-7721-GFP/Luc cells with different concentra- tions were intravenously inoculated into the tail Vein and spleen of the BALB/c-nu/nu mice, the distribu- tion and expression of luciferase in nude mice were monitored by in vivo bioluminescence imaging system. Results The experimental metastatic animal models had been successfully established. The distribution and expression of luciferase ascended with cell concentration increased and decreased with the passage of time. Conclusion The in vivo bioluminescence imaging system may monitor the in vivo growth and metastasis of tumors and provide for studying the mechanisms of tumor metastasis and development of anticancer drug.
作者 闫明霞 朱淼鑫 刘蕾 李静 林河春 赵方瑜 姚明 YAN Ming-xia, ZHU Miao-xin, LIU Lei,Li Jing, Lin He-chun, ZHAO Fang-yu, YAO Ming (Shanghai Cancer Institute, Shanghai 200032, China)
出处 《实验动物与比较医学》 CAS 2012年第5期376-382,共7页 Laboratory Animal and Comparative Medicine
基金 上海市科委创新行动计划(09140900200,11140902100)
关键词 GFP/Luc双标 慢病毒载体 小动物活体成像系统 转移 动物模型 I GFP/Luc double-tagging Lentivirus carrier In vivo bioluminescence imaging system Metastasis Animal model
作者简介 闫明霞(1976-),女,博士,实验肿瘤学。E—mail:mingxiayan@gmail.com 通讯作者 姚明(1959-),男,博士,研究员。研究方向:肿瘤转移模型、转移机理及抗肿瘤实验研究。E—mail:myao@shsci.org
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