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鼠李糖乳杆菌B10通过抗氧化途径改善酒精性肝损伤的研究 预览 被引量:7

Lactobacillus rhamnosus B10 Treatment Ameliorates Ethanol-induced Mouse Liver Injury by Antioxidant Pathways
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摘要 旨在探讨鼠李糖乳杆菌B10对小鼠酒精性肝损伤的改善作用。采用C57BL/6N小鼠,随机分为对照组、酒精组和鼠李糖乳杆菌B10干预组。8周后,下腔静脉取血,分析血清中丙氨酸转氨酶(ALT)、脂多糖(LPS)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。取肝脏进行HE染色和ROS染色,观察肝脏组织病理学变化及氧化损伤的程度,分析肝脏中游离脂肪酸(FFA)、甘油三酯(TG)及胆固醇含量。结果表明:鼠李糖乳杆菌B10显著降低了酒精引起的肝脏脂肪变性及氧化损伤程度(P〈0.05),明显降低了酒精引起的小鼠血清中ALT、LPS和MDA含量(P〈0.05)的升高,使SOD活性显著升高(P〈0.05);明显降低了酒精引起的小鼠肝脏游离脂肪酸(FFA)、TG及胆固醇含量升高(P〈0.05)。提示鼠李糖乳杆菌B10通过抗氧化途径能够减低酒精对小鼠肝脏氧化损伤达到修复酒精性肝损伤的作用。 This study examined whether Lactobacillus rhamnosus B10 treatment can ameliorate ethanol-induced liver injury by antioxidant pathways in a mouse model of alcoholic steatohepatitis.Mice were randomly divided into control group,ethanol treatment group and L.rhamnosus B10 treatment group.After treatment for 8 weeks,blood samples were collected from the inferior vena cava of mice for the determination of serum ALT,LPS,MDA and SOD levels and mouse livers were observed for histopathological features and oxidative injury and analyzed for the contents of FFA(free fatty acids),TG and cholesterol.L.rhamnosus B10 treatment significantly relieved alcohol-induced fatty degeneration and oxidative injury in mice(P0.05),lowered the increase in the levels of serum ALT,LPS and MDA(P0.05),increased SOD activity(P0.05),and inhibited the increase in the levels of liver FFA,TG and cholesterol(P0.05).These findings suggest that L.rhamnosus B10 treatment can ameliorate alcohol-induced liver injury by antioxidant pathways in mice.
作者 王玉华 高晶 张静 胡耀辉 WANG Yu-hua,GAO Jing,ZHANG Jing,HU Yao-hui(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
出处 《食品科学》 CAS CSCD 北大核心 2012年第23期270-273,共4页 Food Science
基金 国家自然科学基金青年科学基金项目(31101252) 长春市科技计划项目(2011111)
关键词 鼠李糖乳杆菌B10 抗氧化 酒精性肝损伤 Lactobacillus rhamnosus B10 antioxidant alcohol-induced liver injury
作者简介 王玉华(1972-),女,副教授,博士,研究方向为食品微生物与功能性食品。E-mail:yuhua.ww@163.com
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