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牙囊细胞对炎症组织来源牙周膜干细胞增殖、成骨和干性作用的影响 预览 被引量:1

Effects of dental follicle cells on the proliferation, osteogenesis and stemness of periodontitis tissue derived periodontal ligament stem cells
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摘要 目的:研究牙囊细胞(Dental follicle cells,DFCs)对炎症组织来源的牙周膜干细胞(peri—odomtitis tissue derived periodontal ligament stemcells,PPDLSCs)增殖、成骨能力和干性作用的影响。方法:利用transwell小室进行DFCs与PPDLSCs共培养,通过克隆形成率和细胞周期实验对共培养的PPDLSCs增殖能力进行检测;成骨诱导实验观察其成骨分化能力;同时利用RT—PCR检测成骨相关基因和干性相关基因的表达水平。结果:和对照组相比,与DFCs共培养后的PPDLSCs克隆形成率增强(P〈0.05),处于增殖期细胞增多;成骨诱导后矿化结节明显增多,成骨相关基因Runx2,OCN,ALP明显上调(P〈0.05);干性相关基因Oct4,Sox2,Klf4的表达也大幅上调(P〈0.05)。结论:与DFCs共培养可以增强PPDLSCs的增殖与成骨分化能力,并增强其干性基因的表达。 AIM: To study the effects of dental follicle cells (DFCs) on the proliferation, osteogenesis and stemness of periodontitis tissue derived periodontal ligament stem cells (PPDLSCs). METHODS : The PPDLSCs and DFCs were co-cultured in transwells with 0.4μm- intersaeptum between chambers. The proliferation, osteogenic dif-ferentiation and stemness of PPDLSCs were examined by colony forming assay and cell cycle analysis, osteogenic induc- tion experiment and RT-PCR, respectively. RESULTS : Compared with controls, the colony forming rate of PPDLSCs co-cuhured with DFCs was higher, and the percentage of cells in G2 + M phase was larger. Meanwhile, there were more mineralized nodules in the co-cultures. PPDLSCs co-cultured with DFCs expressed higher mRNA levels of Runx2, OCN, ALP, Oct4, Sox2 and Klf4. CONCLUSION: DFCs can promote the proliferation, osteogenesis and sternness of PPDLSCs.
作者 刘佳 王丽颖 刘文佳 宋扬 黄闯 金岩 金作林 LIU Jia, WANG Li-ying, LIU Wen-jia, SONG Yang, HUANG Chuang, JIN Yan, JIN Zuo-lin ( School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China)
出处 《牙体牙髓牙周病学杂志》 CAS 北大核心 2013年第3期168-173,共6页 Chinese Journal of Conservative Dentistry
基金 国家自然科学基金(30973358)
关键词 牙周膜干细胞 牙囊细胞 共培养 periodontal ligament stem cells (PDSLCs) dental follicle cells (DFCs) co-culture
作者简介 刘佳(1987-),女,汉族,黑龙江人。硕士生(导师:金作林) 通讯作者:金作林,E-mail:zuolinj@fmmu.edu.cn
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