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脐血移植中移植物抗宿主病低发生机理研究

Mechanism of low GVHD incidence in umbilical cord blood transplantation
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摘要 目的:通过对脐血和外周血免疫功能的比较,探讨了脐血移植中移植物抗宿主病(GVHD)低发生率的分子机理。方法:经增殖试验、NK杀伤活性试验及免疫荧光标记方法研究了4种血清对T细胞免疫功能的影响。结果与成人血清相比较,脐血血清能降低NK细胞杀伤肿瘤靶细胞的活性;同时脐血血清和成人血清都能增加丝裂原特异性T淋巴细胞增殖,成人血清在增殖反应中可达到峰值,而脐血血清没有达到峰值效应,甚至在0.1的稀释度时仍 Objective\ To compare the immunological function between umbilical cord blood (UCB) and peripheral blood, and to explore the mechanism of low incidence of graft\|versus host disease (GVHD) in UCB transplantation. Methods\ The proliferation assay,NK cell killing activity assay and immunologic fluorescence double label technique were used to study four sear effect on T cell immunologic function. Results\ UCB serum could reduce K 562 cell activity, compared to that of adult serum.Furthermore,adult serum will enhanced the percentage of HLA DR expression of T cells on PHA\|stimulated PBMC,whereas fetal serum has no such effect.Both adult and fetal serum enhanced mitogen\|specific T cell proliferation,the peak proliferative response could be reached with adult serum but not with fetal serum. Conclusion\ There must be some soluble factors secreted into serum,which play a role in T cell activation and associate with the incidence of GVHD.\;
作者 张占英 诸雪珍 ZHANG Zhanying,ZHU Xuezhen,ZHANG Lansheng, et al.Department of Radiation Medicine,Soochow University,Suzhou 215007,China\;
出处 《中华放射医学与防护杂志》 CAS CSCD 北大核心 2000年第4期 245-247,共3页 Chinese Journal of Radiological Medicine and Protection
基金 :国家自然科学基金!资助项目 (39970 2 44) 国家核工业基金!资助项目 (Y7197Q6 2 0 6 )
关键词 脐血移植 移植物抗宿主病 低发生病 分子机理 Umbilical cord blood \ T cells \ Soluble factors \ Graft\|versus\|host disease
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参考文献3

  • 1朱寿彭,张澜生..医用同位素示踪技术[M].北京:原子能出版社,1989:222.
  • 2Lee S,Blood,1995年,86期,163页 被引量:1
  • 3朱寿彭,医用同位素示踪技术,1989年,134页 被引量:1
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