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两种方法检测乙型肝炎患者血清HBV-DNA的应用价值 预览 被引量:4

PCR-EB qualitative analysis versus amp-lisensor quantitative assay for detection of HBV-DNA in serum in patients with hepatitis B
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摘要 目的:观察两种不同方法检测乙型肝炎患者血清中的乙型肝炎病毒(hepatitis B virus,HBV)-DNA的应用价值比较.方法:分别使用PCR-EB定性及Amp-lisensor定量法对HBV患者,乙型肝炎表面抗原(hepatitis B virus surface antigen,HBsAg)与抗-HBs同时阳性、HBsAg阳性、抗-HBs阳性、HBsAg与抗-HBs同时阴性进行血清HBV-DNA检查,观察2组患者阳性检测率.结果:B组(HBsAg阳性)患者HBSAg(S/N)值高于A组(P〈0.05),C组抗-HBs值高于A组(HBsAg与抗-HBS同时阳性)(P〈0.05),Amp-lisensor定量检测下,患者HBV-DNA阳性率明显高于PCR-EB定性组(P〈0.05).Amp-lisensor定量检测中,C组(抗-HBS阳性)患者的阳性检测率最低(P〈0.05),乙型肝炎患者血清中H B V-D N A对数值均有明显的上升,与E组(乙型肝炎两对半全阴性)比较均有统计学意义(P〈0.05).A组与B组HBV-DNA对数值明显高于C组与D组(HBsAg与抗-HBs同时阴性)(P〈0.05),A组与B组HBV-DNA对数值比较差异无统计学意义(P〉0.05).结论:Amp-lisensor定量法检测血清HBV-DNA的灵敏度较高,在抗-HBs阳性患者中仍可以检测到HBV-DNA. AIM: To compare the application value of PCR- EB qualitative analysis versus amp-lisensor quantitative assay in the detection of HBV-DNA in serum in patients with hepatitis B. METHODS: PCR-EB qualitative analysis or amp- lisensor quantitative assay was used to detect serum HBV-DNA levels in patients with hepatitis B who were positive for both HBsAg and anti- HBs (group A), those positive for HBsAg alone (group B), those positive for anti-HBs alone (group C), or those negative for both HBsAg and anti- HBs (group D). The detection rate of serum HBV- DNA was compared between the two groups.RESULTS: HBsAg (S/N) value was signifi- cantly higher in group than in group A (P 〈 0.05), and anti-HBs value was significantly higher in group C than in group A (P 〈 0.05). The detec- tion rate of serum HBV-DNA by Amp-lisensor quantitative assay was significantly higher than that by PCR-EB qualitative analysis (P 〈 0.05). For amp-lisensor quantitative assay, the positive rate of serum HBV-DNA was lowest in patients of group C (P 〈 0.05). Serum HBV-DNA rose significantly in patients with hepatitis B. HBV- DNA levels were significantly higher in groups A and B than in groups C and D (all P 〈 0.05), but showed no significant differences between groups A and B (P 〉 0.05). CONCLUSION: Amp-lisensor quantitative assay has a high sensitivity in the detection of serum HBV-DNA and is even able to detect HBV-DNA in patients who are positive for anti-HBs.
作者 叶儒军 郑培锐 区宇洁 魏威 Ru-Jun Ye, Pei-Rui Zheng, Yu-Jie Ou, Wei Wei Ru-Jun Ye, People's Hospital of Liangping County, Chongqing 405211, China Pei-Rui Zheng, Yu-Jie Ou, Ann Gene Co., LTD, Sun Yat- Sen University, Guangzhou 510000, Guangdong Province, China
出处 《世界华人消化杂志》 CAS 北大核心 2013年第21期2109-2112,共4页 World Chinese Journal of Digestology
关键词 PCR-EB Amp-lisensor 乙型肝炎病毒 乙型肝炎病毒脱氧核糖核酸 PCR-EB, Amp-lisensor Hepatitis B vi-rus HBV-DNA
作者简介 叶儒军,主管检验师,主要从事临床检验方面的研究 通讯作者:魏威,讲师,510515,广东省广州市广州大道北183E号,南方医科大学生物技术学院wwei20052005@163.com
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