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黔南烟草马铃薯Y病毒(PVY)株系的分子鉴定 预览 被引量:2

Molecular Identification of Strains of Potato virus Y Derived from Infected Tobacco Plants in Qiannan
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摘要 为了揭示贵州省黔南烟区马铃薯Y病毒(PVY)株系分布及分子变异规律,先利用血清学方法初步鉴定PVY株系,再选择19个PVY分离物进行分子鉴定。结果表明,克隆的19个PVYcDNA片段全长均为1074nts,包含1个801nts的完整cp基因,编码266个氨基酸。序列比对发现,19个黔南PVY分离物cp基因核苷酸序列相似性为87.0%~99.9%,与GenBank登录的其他17个PVY株系cp基因序列相似性为86.6%~99.9%。系统进化树分析表明,黔南烟区19个PVY分离物中,有3个PVY分离物与PVY“株系和PVY”“亲缘关系较近,而其他16个PVY分离物与PVY”。株系的亲缘关系最近。 To reveal the rules of distribution and molecular variation of strains of Potato virus Y (PVY) in Qiannan tobacco belt of Guizhou, PVY strains were initially identified by serological method; further, 19 selected PVY isolates were subjected to molecular identification. The results showed that all the cloned fragments of the 19 PVY cDNA were 1074 nts in full-length, containing a complete cp gene of 801 nts and encoding 266 amino acids. The results of sequence comparison showed that the similarity of nucleotide sequence of cp genes in the 19 PVY isolates was 87.0%-99.9%, and the similarity with the cp genes in the 17 PVY strains logged in GenBank was 86.6%-99.9%. Phylogenetic tree analysis indicated that 3 of the 19 PVY isolates from Qiannan had closer relationship with PVYN and PVYNTN, and the other 16 PVY isolates were more closely related to PVYN :o
作者 陆新莉 钱凤英 黄刚 苟正贵 陈威霖 王林玉 黎明江 刘书凯 朱显灵 江彤 LU Xinli% QIAN Fengying', HUANG Gang', GOU Zhenggui% CHEN Weilinl, WANG Linyu, LI Mingjiangl, LIU Shukai', ZHU Xianling2, and JIANG Tong.3 1. Qiannan Tobacco Company of Guizhou Province, Duyun 558000, Guizhou, China 2. Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230051, China 3. College of Plant Protection, Anhui Agricultural University, Hefei 230036, China
出处 《烟草科技》 EI CAS 北大核心 2013年第8期71-74,共4页 Tobacco Science & Technology
基金 基金项目:贵州省黔南州烟草公司资助项目“黔南烤烟良好农业规范体系建设及其应用研究”[黔南科技发(2011)30号] “烟草马铃薯Y病毒病发生规律及防控技术研究”[黔南科技发(2010)9号].
关键词 烟草 马铃薯Y病毒 株系 CP基因 分子鉴定 Tobacco Potato virus Y Strain ep gene Molecular identification
作者简介 陆新莉(1970-),女,学士,农艺师,主要从事烟草病虫害防治研究。E-mail:8258690@qq.com;
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