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杉木与台湾杉EST-SSR标记的开发与应用 预览 被引量:1

Development and Application of EST-SSR Markers for Cunninghamia lanceolata and Taiwania cryptomerioides
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摘要 简单序列重复(Simple Sequence Repeats,SSR)标记是基于SSR中核苷酸单位串联重复次数不同而开发的一种分子标记技术,具有多态性丰富、呈共显性遗传、易于PCR检测,在基因组上分布均匀等特点(Powell et al.,1996)。直接利用现有的EST(Expressed Sequence Tag)序列开发SSR标记就是目前采用较多的一种策略。 Cunninghamia lanceolata is one of the most important fast-growing timber tree species in southern China. In order to develop an effective molecular maker,which is important for genetic diversity analysis and marker-assisted selection in C. lanceolata,all the EST sequences of C. lanceolata and Taiwania cryptomerioides were obtained from NCBI for the development of EST-SSR markers. As a result,311 non redundant EST sequences were assembled from 408 EST sequences of C. lanceolata. Among these sequences,a total of 28 SSRs were identified from 26 non redundant EST sequences,and the trinucleotide and tetranucleotide repeats were the dominant types with the frequency of 60. 71% and25%,respectively. A total of 384 non redundant EST sequences were found after assembly of 2 643 EST sequences of T.cryptomerioides and 32 SSRs were identified from 27 non redundant EST sequences. The trinucleotide and tetranucleotide repeats were also the dominant types with the frequency of 53. 13% and 25%,respectively. According to these sequences,50 pairs of EST-SSR primers were designed. Through PCR and sequencing,10 pairs of primers with polymorphism were isolated,among which,9 pairs of primers were from C. lanceolata and 1 pair of primers was from T. cryptomerioides. These polymorphic primers were further used to assess the genetic diversity of 30 different clones of C. lanceolata. The results showed that 30 clones were divided into 6 groups at coefficient rate 0. 80. All the results indicated that the developed SSR markers from EST sequences would have important application value in genetic analysis and molecular breeding of C.lanceolata.
作者 张圣 黄华宏 林二培 童再康 Zhang Sheng;Huang Huahong;Lin Erpei;Tong Zaikang;Nurturing Station for the State Key Laboratory of Subtropical Silviculture Zhejiang A&F University;
出处 《林业科学》 CAS CSCD 北大核心 2013年第10期173-180,共8页 Scientia Silvae Sinicae
基金 浙江省重点科技创新团队(2009R50035) 国家863项目(2011AA100203) 浙江省科学技术厅优先主题重点农业项目(2011C12014)
关键词 杉木 台湾杉 EST SSR 遗传多样性 Cunninghamia lanceolata Taiwania cryptomerioides EST SSR genetic diversity
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  • 1Altschul S F, Gish W, Miller W, et al. 1990. Basic local alignmentsearch tool. Journal of Molecular Biology, 215 (3) : 403 - 410. 被引量:1
  • 2B6rub6 Y, Zhuang J, Rungis D, et al. 2007. Characterization of EST- SSRs in loblolly pine and spruce. Tree Genetics and Genomes, 3 (3): 251 -259. 被引量:1
  • 3Blacket M J, Robin C, Good R T, et al. 2012. Universal primers for fluorescent labelling of PCR fragments-an efficient and cost- effective approach to genotyping by fluorescence. Molecular Ecology Resources, 12(3) : 456 -463. 被引量:1
  • 4Br/tutigam A, Mullick T, Schliesky S, et al. 2011. Critical assessment of assembly strategies for non-model species mRNA-Seq data and application of next-generation sequencing to the comparison of C3 and C4 species. Journal of Experimental Botany, 62(9) : 3093 - 3102. 被引量:1
  • 5Buschiazzo E, Gemmell N J. 2006. The rise, fall and renaissance of microsatellites in eukaryotic genomes. BioEssays, 28 (10): 1040- 1050. 被引量:1
  • 6Castoe T A, Poole A W, de Koning A P J, et al. 2012. Rapid microsatellite identification from Illumina paired-end genomie sequencing in two birds and a snake. PLoS ONE, 7(2) : e30953. 被引量:1
  • 7Chung J D, Lin T P, Tan Y C, et al. 2004. Genetic diversity and biogeography of Cunninghamia konishii (Cupressaceae) , an island species in Taiwan: a comparison with Cunninghamia lanceolata, a mainland species in China. Molecular Phylogeneties and Evolution, 33(3) : 792 -801. 被引量:1
  • 8da Maia L C, Palmieri D A, de Souza V Q, et al. 2008. SSRLoeator: tool for simple sequence repeat discovery integrated with primer design and PCR simulation. International Journal of Plant Genomics, doi: 10. 1155/2008/412696. 被引量:1
  • 9Davey J W, Hohenlohe P A, Etter P D, et al. 2011. Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nature Reviews Genetics, 12(7) : 499 -510. 被引量:1
  • 10Duran C, Singhania R, Raman H, et al. 2013. Predicting polymorphic EST-SSRs in silico. Molecular Ecology Resources, 13 (3) : 538 - 545. 被引量:1

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