期刊文献+

痛风性关节炎患者血清白细胞介素-1β肿瘤坏死因子-α环氧化酶-2水平的动态变化研究 被引量:25

The dynamic changes of the serum levels of interleukin-1β, tumor necrosis factor-α cyclooxygenase-2 in acute gouty arthritis patients
收藏 分享 导出
摘要 目的观察严重程度不同的急性痛风患者血清白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α、环氧化酶-2的动态变化,为确定痛风急性期镇痛药物的使用疗程提供依据。方法选择痛风性关节炎患者90例,其中急性发作期60例,缓解期30例(观察组3),健康体检者(对照组)30名。参照主诉疼痛程度评分法(VRS-4法),将急性痛风性关节炎分为重度(观察组1)和轻度(观察组2)2个亚组。痛风急性发作期的患者均给予相同剂量和疗程的依托考昔和秋水仙碱治疗14d,应用酶联免疫吸附法(ELISA)分别检测发病后第1、3、7、10、14天血清IL-1β、TNF-α、环氧化酶.2水平。组间比较采用t检验,多组间均数比较采用单因素方差分析。结果①与对照组相比,观察组1、2在急性痛风性关节炎发病的第1~7天IL-1B、TNF-α水平明显升高(P〈0.01),第10-14天恢复正常水平;环氧化酶-2水平在第1~3天明显升高(P〈0.01),第7-10天恢复正常水平;而上述指标观察组3与对照组比较差异无统计学意义(t=-0.880,-1.201,-0.548;P=0.383,0.235,0.586)。②与观察组2相比,在急性痛风发病的第10天观察组1 IL-1β、TNF-α水平明显升高[(24±5)pg/ml和(19±3)pg/ml,(323±84)ng/ml和(234±29)ng/ml;P=0.001,0.002],而观察组2与对照组之间比较差异无统计学意义(P=0.357);环氧化酶-2水平在急性痛风发病的第7天观察组1明显升高[(12.9±2.0)pg/ml和(9.1±1.6)pg/ml],而观察组2与对照组之间比较差异无统计学意义(P=0.941)。结论急性痛风性关节炎镇痛治疗10-14d炎性因子才能完全恢复正常。本研究为急性痛风性关节炎镇痛药物使用疗程提供了依据。 Objeαive To investigate the dynamic changes of serum interleukin (IL)-1β, tumor necrosis faαor (TNF)-α, cyclooxygenase (COX)-2 levels in patients with acUte gouty arthritis with various severityes and to provide evidence for the course of anti-inflammatory drug used in patients with acute gouty arthritis. Methods Ninety patients with gouty arthritis were randomly seleαed including sixty acute gouty arthritis patients and thirty gout patients in remission (observation group 3). Sixty acute gouty arthritis patients were divided into severe (observation group 1) and mild (observation group 2) subgroups according to the chief complaints and pain score points (VRS-4 method). All acute gouty arthritis patients were given the same dose and duration of etoricoxib and colchicine (14 days in all). Serum IL-1β, TNF-α, COX-2 levels were deteαed by enzyme-linked immunosorbent assay (ELISA) after the 1, 3, 7, 10 and 14 days after onset. The 2-tailed independent samples t test was used for the analysis of measurement data. ANOVA were adapted for the comparison between groups. Results ①The serum levels of IL-1β, TNF-α in the observation group 1, 2 were significantly higher than those in the control group in day 1 and 7 (P〈0.01), and almost completely returned to normal at day 10 to 14 day anti-inflammatory therapy. The serum COX-2 levels in observation group 1, 2 were significantly higher than those in the control group (P〈0.01) in day 1 to 3, and returned to normal at day 7 to 10 after anti-inflammatory therapy. There were no significant differences between observa-tion group3 and the control group (t=-0.880, -1.201, -0.548; P=0.383, 0.235, 0.586).② The serum levels of IL-1β, TNF-α were significantly higher in observation group 1 than those in observation group 2 [ (24±5) pg/ml and(19±3) pg/ml, (323±84) ng/ml and (234±29) ng/ml; P=0.001, 0.002], while there was no signi-ficant differences between observation group 2 and the control group (
作者 袁艳平 李长贵 YUAN Yah-ping, LI Chang-gui. (Department of Medicine, the Affiliated Hospital of Medical College of Qingdao University, Shandong 266003, China)
出处 《中华风湿病学杂志》 CAS CSCD 北大核心 2013年第12期818-822,共5页 Chinese Journal of Rheumatology
关键词 白细胞介素1 肿瘤坏死因子α 前列腺素内过氧化物合酶 关节炎 痛风性 Interleukin-1 Tumor necrosis factor-alpha Prostaglandin-endoperoxide synthase Arthritis, gouty
作者简介 通信作者:李长贵,Email:lichanggui@medmail.com.cn
  • 相关文献

参考文献16

二级参考文献161

  • 1周敏,李萌,韩玉生,赵松,杨伟鹏.痹清胶囊对痛风性关节炎大鼠关节滑膜组织中5-羟色胺、钾离子含量的影响[J].中国实验方剂学杂志,2008(12):47-48. 被引量:3
  • 2姜宏 ,惠祁华 ,陈咏真 ,曹莉 .痛风平对实验性急性痛风性关节炎抗炎消肿镇痛作用的研究[J].中国中医药信息杂志,2005,12(3):30-32. 被引量:8
  • 3姜贵云,杨晓莲.痛风病的诊断评估与临床特征[J].中国临床康复,2006,10(28):150-155. 被引量:30
  • 4Dahlberg L,Billinghurst RC,Manner P,et al.Selective enhancement of collagenase-mediated cleavage of resident type II collagen in cultured osteoarthritic cartilage and arrest with a synthetic inhibitor that spares collagenase 1 (matrix metalloproteinase 1)[J].Arthritis Rheum,2000,43(3):673-682. 被引量:1
  • 5Abbaszade I,Liu RQ,Yang F,et al.Cloning and characterization of ADAMTS11,an aggrecanase from the ADAMTS family[J].J Biol Chem,1999,274(3):23443-23450. 被引量:1
  • 6Tortorella MD,Burn TC,Pratta MA,et al.Purification and cloning of aggrecanase-1:a member of the ADAMTS family of proteins[J].Science,1999,284(5420):1664-1666. 被引量:1
  • 7Hussein MR,Fathi NA,El-Din AM,et al.Alterations of the CD4(+),CD8 (+) T cell subsets,interleukins-1beta,IL-10,IL-17,tumor necrosis factor-alpha and soluble intercellular adhesion molecule-1 in rheumatoid arthritis and osteoarthritis:preliminary observations[J].Pathol Oncol Res,2008,14(3):321-328. 被引量:1
  • 8Joosten LA,Helsen MM,Saxne T,et al.IL-1 alpha beta blockade prevents cartilage and bone destruction in murine type II collagen-induced arthritis,whereas TNF-alpha blockade only ameliorates joint inflammation[J].J Immunol,1999,163(9):5049-5055. 被引量:1
  • 9Zwerina J,Redlich K,Polzer K,et al.TNF-induced structural joint damage is mediated by IL-1[J].P Natl Acad Sci USA,2007,104(28):11742-11747. 被引量:1
  • 10Livak KJ,Schmittgen TD.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J].Methods,2001,25(4):402-408. 被引量:1

共引文献163

同被引文献281

引证文献25

二级引证文献92

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈