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高压氧对Aβ25—35诱导大鼠认知和记忆障碍及其海马神经元凋亡的影响 被引量:2

A possible anti-apoptosis mechanism of hyperbaric oxygen in rats with memory impairments induced by Aβ25-35
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摘要 目的探讨高压氧(HBO)治疗对β-淀粉样蛋白(Aβ)25—35所致拟阿尔茨海默病(AD)模型大鼠认知和记忆功能的改变及其海马神经元凋亡情况的影响。方法选取健康成年雄性SpragueDawley(SD)大鼠48只,按随机数字表法分为正常对照组、假手术组、模型组和HBO治疗组,每组12只。正常对照组不做任何处理。其余各组大鼠给予10%水合氯醛(4m1)腹腔注射麻醉,假手术组大鼠每侧海马注射5μl生理盐水;造模大鼠(模型组和HBO治疗组)每侧海马注射5μl的A325—35制备拟AD大鼠痴呆模型。造模成功后,模型组大鼠不做任何治疗处理;HBO治疗组大鼠造模2周后,常规HBO治疗,每日1次,10d为1个疗程,中间休息3d,共2个疗程。采用Morris水迷宫法观察各组大鼠空间记忆能力的改变,TUNEL染色观察大鼠海马神经元凋亡情况的改变,同时检测海马组织凋亡相关基因Bcl-2和Bax的mRNA、蛋白表达的改变。结果水迷宫实验中,第5天和第6天HBO治疗组大鼠的逃避潜伏期与模型组比较显著缩短(33.4±4.5)s比(48.1±2.7)s,(20.8±1.7)s比(40.5±1.9)S,P〈0.05,空间探索实验中HBO治疗组大鼠在原平台所在象限的时间及穿过原平台的次数较模型组显著增加(35.8±5.6)%比(21.1±3.8)%,(4.8±1.1)次比(3.1±1.2)次,P〈0.05。TUNEL染色中,模型组海马神经元中胞核呈现棕色凋亡形态的较多,而HBO治疗组则见少数凋亡的海马神经元。海马组织凋亡基因检测中,HBO治疗组Bcl-2mRNA和蛋白的表达显著高于模型组(P〈0.05),其BaxmRNA和蛋白的表达则相应地降低。结论HBO可能通过抑制Aβ25—35诱导的海马神经元凋亡而改善AD大鼠模型的认知和记忆能力。 Objective To explore the possible protective effect of hyperbaric oxygen (HBO) on cogni- tive deficits induced by amyloid β25-35 ( Aβ25-35 ) and neuronal apoptosis in the hippocampi of rats with Alzheimer's disease (AD). Methods The animal AD model was established in 24 Sprague-Dawley rats by bilateral hippocampal injection of Aβ25-35. Twelve rats were injected with normal saline as controls, and anoth- er 12 served as normal controls. After the injection, the model rats were further divided into a model group and a treatment group. All the rats were housed with normal feeding for 2 weeks and then those in the treatment groups received a total of 2 courses of HBO treatment ( 10 days each with an interval of 3 days in between). The other groups were left with no treatment. After the treatment, the rats' learning and memory ability were tested u- sing Morris' water maze test, and any neuronal changes were observed using TUNEL staining. The expression of mRNA and Bcl-2 and Bax proteins in the hippocampus were detected using a RT-PCR and Western blotting. Results HBO significantly improved the learning and memory impairment and alleviated neuronal apoptosis in the hippocampus compared against the control group. In addition, HBO treatment significantly increased the mRNA and protein expression of Bcl-2 and down-regulated the expression of Bax. Conclusion HBO treatment can prevent learning and memory impairment induced by Aβ25-35 peptides, which might be mediated by inhibi- ting neuronal apoptosis in the hippocampus.
作者 田小强 张丽 杨琳 黄平 钱霞 黄培林 张丽达 Tian Xiaoqiang*, Zhang Li, Yang Lin, Huang Ping, Qian Xia, Huang Peilin, Zhang Lida. Department of Oncology, The Second Affiliated Hospital of Southeast University, Nanjing 210009, China
出处 《中华物理医学与康复杂志》 CSCD 北大核心 2014年第1期7-11,共5页 Chinese Journal of Physical Medicine and Rehabilitation
基金 南京市社会发展科技计划(200804039) 南京市医学科技发展重点项目资助(ZKX13034)
关键词 阿尔茨海默氏病 高压氧 Β-淀粉样蛋白 细胞凋亡 Alzheimer's disease Hyperbaric oxygen β-amyloids Apoptosis
作者简介 通信作者:张丽达,Email:13951940316@163.com
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参考文献11

  • 1Vickers JC, l)icksml TC, Adlard PA, et genera/ion in Alzheimer's disease[ J] al. The cause of neuronal de- Prog Neurobiol, 2000,60 ( 2 ) : 139-165. 被引量:1
  • 2Pan YF,Chen XR, Wu MN ,et al. Arginine vasopressin prevents againstAbeta(25-35 )-induced impairment uf spatial learning and memory in rats[ J ]. Horm Behav ,2010,57 ( 4-5 ) :448-454. 被引量:1
  • 3Klementiev B, Novikova T, Nnvitskaya V ,el al. A neural cell adhesion molecule-derived peptide reduces neuropathological signs and cognitive impairment induced by Abeta25-35 [ J]. Neuroscieuce,2007,145 ( I ) : 209 -224. 被引量:1
  • 4Stepanichev M Y, Zdobnova IM, Zarubenku II, et ai. Amyluid-beta ( 25- 35)-induced memory impairments correlate with cell toss in rat hippu- campus[J]. Physiol Behav,2004,80( 5 ) :647-655. 被引量:1
  • 5Chauhan V ,Chauhan A. Oxidative stress in Alzheimer's disease[ J]. Pathuphysiology ,2006,13 ( 3 ) : 195-208. 被引量:1
  • 6Praticb D. Oxidative stress hypothesis in A lzheimer's disease:a reap- praisal [ J ]. Trends Pharmacul Sei,2008,29 ( 12 ) :609-615. 被引量:1
  • 7Bastianetto S, Ramassamy C, Dor6 S, el al. thie (,inkgo lliloba extract (EGb 761 ) protects hippocampal neurons against cell death induced by beta-amyloid[ J]. Eur J Neurosei ,2000,12 ( 6 ) : 1882-1890. 被引量:1
  • 8Wang YC,Zhang S,Dn TY,et al. ttBO precunditinning reduces ische- mia-reperfnsiun injury by stimulating autuphagy in ncurot.ytc[ J ]. Brain Res,2010,1323(1) :149-151. 被引量:1
  • 9Wang GH ,Zhang XG ,Jiang ZL,el al. Neuroprotec'tiw~ effects of hyper- baric oxygen treatment on traumatic brain injuu,' in the rat[ J ]. J Neuro- trauma, 201 O, 27 ( 9 ) : 1733-1743. 被引量:1
  • 10宋娟,高晓平.脑缺血致学习记忆功能障碍机制的研究进展[J].中华物理医学与康复杂志,2012,34(1):67-69. 被引量:2

二级参考文献33

  • 1王彤,于建春,刘存志,姜文,熊会海,邢海涛,韩景献(指导).针刺对MID大鼠病理形态及空间学习记忆的影响[J].上海针灸杂志,2006,25(10):44-47. 被引量:1
  • 2Nikonenko AG, Radenovic L, Andjus PR, et al. Structural features of ischemic damage in the hippocampus. Anat Rec, 2009,292: 1914- 1921. 被引量:1
  • 3Pires VL, Souza JR, Cuimarass SB, et al. Preconditioning with L-alanyl-L-glutamine in a Mongolian gerbil model of acute cerebral ischemia/reperfusion injury. Acta Cir Bras,2011 ,26: 14-20. 被引量:1
  • 4Leuner B, Gould E. Structural plasticity and hippocampal function. Annu Rev Psychol ,2010 ,61: 111-113. 被引量:1
  • 5Deng W, Aimone JB, Gage FH. New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory? Nat Rev Neurosci, 2010,11: 339-350. 被引量:1
  • 6Deshmukh SS, Yoganarasimha D, Voicu H, et al. Theta modulation in the medial and the lateral entorhinal cortices. J Neurophysiol, 2010, 104 :994-1006. 被引量:1
  • 7Manns JR, Eichenbaum. A cognitive map for object memory in the hippocampus. Learn Mem,2009,16:616-624. 被引量:1
  • 8Sahay A, Hen R. Hippocampal neurogenesis and depression. Novartis Found Symp,2008 ,289: 152-160. 被引量:1
  • 9Richter JD, Klann E. Making synaptic plasticity and memory last: mechanisms of translational regulation. Genes Dev , 2009,23: 1-11. 被引量:1
  • 10Lynch MA. Long-term potentiation and memory. Physiol Rev ,2004,84: 87-136. 被引量:1

共引文献1

同被引文献33

  • 1吴冰洁,顾平,崔冬生,耿媛,王铭维.丰富环境对快速老化小鼠SAMP8学习记忆能力的影响[J].第二军医大学学报,2007,28(9):964-967. 被引量:7
  • 2de Magalhaes JP, Wuttke D, Wood SH, et al.Genome-environment in- teractions that modulate aging: powerful targets for drug discovery [ J ]. Pharmacol Rev, 2012,64( 1 ) : 88-101. 被引量:1
  • 3Hu YS, Xu P, Pigino G, et al. Complex environment experience res- cues impaired neurogenesis, enhances synaptic plasticity, and attenu- ates neuropathology in familial Alzheimer' s disease-linked APPswe/ PS1Deha E9 mice[ J] .FASEB J,2010,24(6) : 1667-1681. 被引量:1
  • 4Yuan ZY, Gu P, Liu L, et al. Neuroprotective effects of enriched envi- ronment in MPTP-treated SAMP8 mice [ J ]. Neurosci Lett, 2009,454 ( 1 ) :6-10. 被引量:1
  • 5Kang L,Li S,Xing ZG,et al. Dihydrotestosterone treatment delays theconversion from mild cognitive impairment to Alzheimer's disease in SAMP8 mice[J].Horm Behav,2014,65 (5) :505-515. 被引量:1
  • 6Zilkova M,Koson P,Zilka N. The hunt for dying neurons:insight into the neuronal loss in Alzheimer' s disease [ J ]. Bratisl Lek Listy, 2006, 107(9-10) :366-373. 被引量:1
  • 7van Praag H, Kempermann G, Gage FH. Neural consequences of envi- ronmental enrichment[ J] .Nat Rev Neurosci ,2000,1 (3) : 191-198. 被引量:1
  • 8Brown J, Cooper-Kuhn CM, Kempermann G, et al. Enriched environ- ment andphysical activity stimulate hippocampal but not olfactory bulb neurogenesis[J] .The Eur J Neurosci,2003,17(10) : 2042-2046. 被引量:1
  • 9Kobilo T, Liu QR, Gandhi K, et al. Running is the neurogenic and neurotrophic stimulus in environmental enrichment [J]. Learn Mem, 2011,18(9) :605-609. 被引量:1
  • 10Herring A, Ambree O, Tomm M, et al. Environmental enrichment en- hances cellular plasticity in transgenic mice with Alzheimer-like pa- thology [ J ]. Exp Neurol, 2009,216 ( 1 ) : 184-192. 被引量:1

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