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牡丹皮对AGEs诱导的系膜细胞增殖及基底膜增厚的影响 被引量:11

Effect of Moutan Cortex on AGEs-induced mesangial cell proliferation and basement membrane thickening
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摘要 目的:探讨牡丹皮对晚期糖基化终产物(advanced glyeation end products,AGEs)诱导的系膜细胞增生及基底膜增厚的影响。方法:建立AGEs诱导的肾小球系膜细胞(MC)损伤模型,设置空白组[牛血清白蛋白(bovine serum albumin,BSA),200mg·L-1],模型组(AGEs,200mg·L-1),氨基胍(aminoguanidine,AG)阳性组(10mmol.L-1),牡丹皮高、中、低剂量组(2×10^-4,1×10^-4,0.5×10^-4g·mL-1)。采用四甲基偶氮唑蓝(MTT)法观察牡丹皮对损伤的MC的增殖影响;采用酶联免疫吸附测定法(ELISA)测定细胞上清液中纤维连接蛋白(fibronectin,FN)和Ⅳ胶原蛋白(type IV collagen secretion,ColIV)的含量;采用Western blotting观察FN的表达;Real—time PCR检测Col IV的mRNA表达水平。结果:牡丹皮能抑制AGEs刺激的MC增殖以及FN和ColIV分泌;Western blotting表明牡丹皮下调MC分泌的FN表达;Real-time PCR结果说明牡丹皮对AGEs刺激下的MC分泌ColIV的mRNA表达有下调作用。结论:牡丹皮对AGEs培养条件下的MC具有一定的保护作用,牡丹皮能明显抑制基质中ColIV及FN的合成与分泌,抑制基底膜增厚,为糖尿病肾病的治疗提供实验依据。 Objective: To investigate the effect of Moutan Cortex on mesangial proliferation and basement membrane thickening induced by advanced glycation end products (AGEs). Method: The glomerular mesangial ceils (MC) injury model was established by inducing by AGEs. The cell were divided into 6 groups : the blank group ( BSA, 200 mg . L- 1 ), the model group ( AGEs, 200 mg . L-1 ), the positive control group (AG, 10 mmol . L-1), and drug administration groups, namely the Moutan Cortex-treated high-dose group (2 × 10 ^-4 g . mL^-1 ), the Moutan Cortex-treated medium-dose group ( 1 × 10^ -4 g . mL-1 ), and the Moutan Cortex-treated lowdose group (0. 5 × 10 ^-4 g . mL-1 ). The MTT method was performed to observe the effect of Moutan Cortex on the proliferation of MC. The content of fibronectin (FN) and collagen secretion IV ( Col IV ) in cell supernatant were detected by ELISA kits. The western blot analysis was carried out to observe the FN expression. The Real-time PCR analysis was applied to examine the Col IV mRNA expression. Result: AGEs significantly increased AGEs-induced MC proliferation and FN and Col IV secretion. The western blot analysis showed that MC could down-regulate the FN expression of MC secretion. According to the results of the real-time PCR assay, MC could down-regulate AGEs-induced MC secretion Col IV mRNA expression. Conclusion: MC had a certain protective effect on MC cultured under AGEs conditions. MC could remarkably inhibit the composition and secretion of Col IV and FN in matrix and the basement membrane thickening, and provide an experimental basis for the treatment of diabetic nephropathy.
作者 张明华 封亮 顾俊菲 蒋俊 贾晓斌 ZHANG Ming-hua, FENG Liang , GUN Jun-fei, JIANG Jun , JIA Xiao-bin (1. School of Pharmacy, Jiangsu University, Zhenjiang 212013, China; 2. Key Laboratory of Drug Delivery System of Chinese Materia Medica under State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China)
出处 《中国中药杂志》 CAS CSCD 北大核心 2014年第3期478-482,共5页 China Journal of Chinese Materia Medica
基金 [基金项目]国家自然科学基金项目(81202906) 江苏省自然科学基金项目(BK2012491)
关键词 牡丹皮 晚期糖基化产物 系膜细胞 基底膜增厚 纤维连接蛋白 IV胶原蛋白 Moutan Cortex advanced glycation end product mesangial cell basement membrane thickening fibronectin collagen secretion IV
作者简介 [作者简介]张明华,硕士研究生,E-mail:jdyx0701.111@163.com. [通信作者]封亮,E-mail:wenmoxiushi@163.com; 贾晓斌,教授,研究员,博士生导师,Tel:(025)85608672,E-mail:jxiaobin2005@hotmail.com
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