Objective： To investigate the biocompatibility between in vitro cultured Schwann cells（SCs） and allogeneic bone scaffolds. Methods： Allograft bone scaffold materials were prepared from fresh human bones and the physical properties were tested. SCs were isolated from extracted sciatic and brachial plexus from newborn SD rats. Two culture conditions were used： three-dimensional（SCs plus allogeneic bone scaffold） and two-dimensional culture model（SCs plus collagen glass）. The purity of SCs was identified with immunofluorescence staining by anti-S-100 antibody. The cells were evaluated for proliferation by cell counting and also imaged with scanning electron microscopy. Results： Allogeneic bone scaffolds had the special three-dimensional pore structure, which supported the adhesion and growth of SCs. The purity of SC was 〉95%. SCs cultured in both three-dimensional and two-dimensional models proliferated well at the similar rate and shared the similar distribution characteristics. Compared with the two-dimensional model, the cells cultured in the three-dimensional model showed slender cell bodies and more cellular pseudopods at the early stage of incubation, and enhanced capability of migration at the later stage of incubation. Conclusions： Allogeneic bone scaffold and SCs have good biocompatibility and the in vitro model of tissue engineering neural bone is successfully established.
Progress in Modern Biomedicine
The nervalization of bone tissue engineering