期刊文献+

亚洲日月蛤多糖的分子检测及其干预小鼠日本血吸虫肝纤维化的初步研究

Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum
分享 导出
摘要 目的检测亚洲日月蛤多糖的分子特性,探讨其在干预小鼠日本血吸虫肝纤维化中的作用。方法提取并纯化亚洲日月蛤多糖,采用凝胶排阻法对其分子量进行测定,应用PMP柱前衍生法对其单糖组成进行检测。取50只雌性BALB/c小鼠,随机分成5组,每组10只小鼠。A组给予生理盐水灌胃,B、C、D、E组小鼠分别经腹部皮肤攻击感染血吸虫尾蚴(30±2)条/只;饲养第8周末,C、D、E组小鼠分别给予多糖、吡喹酮及多糖+吡喹酮治疗,B组小鼠给予生理盐水;第16周末,收集小鼠肝脏及血清,通过HE染色观察小鼠肝脏虫卵肉芽肿病变及肝纤维化情况,采用ELISA法检测血清IFN-γ、IL-13水平。结果亚洲日月蛤多糖相对分子量为11.7kDa,其多糖主要由葡萄糖组成。C、D组和E组小鼠血清IFN-γ浓度显著高于对照组(F=63.525,P<0.01),C、D组和E组小鼠血清IL-13浓度显著低于B组(F=99.788,P<0.01)。HE染色显示,B、C、D、E组小鼠肝组织均见不同程度虫卵结节和纤维化改变,A组小鼠肝脏正常,E组虫卵结节较B组少(x^2=7.875,P<0.05)。结论亚洲日月蛤多糖具有明显的抗日本血吸虫肝纤维化作用,与吡喹酮联用效果更好。 Objective To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes,so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection. Methods The crude polysaccharide from A. pleuronectes was extracted and further purified,and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre.column derivatization method,re.spectively. A total of 50 female BALB/c mice were randomly divided into five groups:A(normal group),B(experimental group),C(polysaccharide group),D(praziquantel),and E(polysaccharide + praziquantel group). The mice in B,C,D,or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum(30 ± 2 for each mouse)respectively. After 8 weeks,the mice in C,D,and E groups were administrated by polysaccharide and/or praziquantel,and the mice in B group were instead of saline. All the livers and sera were collected after 16 weeks. HE staining was employed for the livers,and serum IFN.γ and IL.13 were measured by using ELISA kits. Results The molecular weight of purified polysaccharide from A. pleuro.nectes was 11.7 kDa. Compared with A and B groups,the serum levels of IFN.γ in C,D,and E groups were significantly in.creased(F = 63.525,P < 0.01). However,the serum levels of IL.13 in C,D,and E groups were significantly decreased(F =99.788,P < 0.01)compared with that in B group. HE staining showed that the egg nodules and hepatic fibrosis were observed in B,C,D,and E groups. The number of egg nodules and fibrosis degree in E group were milder than those in B group(c2 =7.875,P < 0.05). Conclusions The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection,particularly combining with the administration of praziquantel.
作者 吕业超 唐小牛 胡伟 姜玉新 湛孝东 郭伟 孙启山 王国栋 周书林 LU Ye-chao;TANG Xiao-niu;HU Wei;JIANG Yu-xin;ZHAN Xiao-dong;GUO Wei;SUN Qi-shan;WANG Guodong;ZHOU Shu-lin(Department of Medical Parasitology,Wannan Medical College,Wuhu 241002,China;Anhui Provincial Engineering Research Center for Polysaccharide Drugs,China)
出处 《中国血吸虫病防治杂志》 CSCD 北大核心 2018年第5期500-503,517共5页 Chinese Journal of Schistosomiasis Control
基金 安徽省高校自然科学重点基金(KJ2018A0263) 国家自然科学基金(81172790、81671586) 皖南医学院第二批学术与技术带头人资助项目(010202041703).
关键词 亚洲日月蛤 多糖 日本血吸虫 肝纤维化 吡喹酮 小鼠 Amusium pleuronectes Polysaccharide Schistosomajaponicum Liver fibrosis Praziquantel Mouse
作者简介 吕业超,男,硕士研究生。研究方向:天然产物与抗感染免疫;通信作者E-mail:txniu@163.com
  • 相关文献

参考文献13

二级参考文献109

共引文献149

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈