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三明野生蕉抗寒特性的形态学与生理生化研究 预览

A study on the phenotypical,physiological and biochemical characteristics of the cold resistant Sanming wild banana(Musa itinerans)
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摘要 [目的]探究三明野生蕉(Musa itinerans)抗寒的形态学和生理生化机制。[方法]以三明野生蕉为材料,以不耐寒‘天宝蕉’(Musa acuminata‘Tianbaojiao’)为对照,采用石蜡切片法对比二者的叶片组织结构和在4℃低温处理24 h前后的表型变化。同时,比较低温处理前后叶片叶绿素荧光参数,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)和多酚氧化酶(PPO)活性,丙二醛(MDA)、脯氨酸(Pro)和可溶性糖(SS)含量的变化差异。[结果]三明野生蕉海绵组织较薄,叶片细胞结构紧密度(CTR)更高。低温处理后,三明野生蕉叶片挺立、无损伤,叶绿素荧光参数均处于较高水平,冷害对其光合系统损伤不明显;低温处理前后,三明野生蕉SOD、POD和PPO活性和Pro含量均显著高于‘天宝蕉’。低温会导致三明野生蕉PPO活性和MDA含量显著降低,而‘天宝蕉’则表现出相反的趋势。室温条件下,三明野生蕉CAT活性与‘天宝蕉’差异不大,低温处理后却显著高于‘天宝蕉’。[结论]三明野生蕉在低温处理前后的表型和叶绿素荧光参数变化不大,推测三明野生蕉抗寒特性与较高的CTR、抗氧化酶活性和Pro含量有关。 [Objective]Banana plants are generally low in cold resistance,and thus often suffer great yield losses in some cultivation regions in south China,such as Guangdong,Guangxi and Fujian provinces due to the chilling or freezing injuries.Fujian has rich wild banana resources,among which the Sanming wild banana(Musa itinerans)has been identified as the most cold-resistant one.The semi-lethal temperature for tissue cultured Sanming wild banana seedlings is -1.776 ℃,which is about 1 ℃lower than most of the cultivated bananas.The clarification cold resistance mechanism of Sanming wild banana will undoubtedly provide a solid basis for the improvement of banana cold resistance,which is of great significance for reducing the loss of banana farmers and sustainable development of local banana industry.However,up to now,the mechanism is still unclear.In the present study,experiments were performed to reveal the cold resistance mechanism of Sanming wild banana.[Methods]To explore the phenotypical,physiological and biochemical characteristics that might contribute to the cold resistance of Sanming wild banana,2-month-old plants were used,and the main banana variety of Fujian province‘Tianbaojiao’(Musa acuminata),a cold susceptible banana variety,was used as the control.We first compared the leaf tissue structures of Sanming wild banana and‘Tianbaojiao’using conventional paraffin sections.Then,they were subjected to low temperature treatment(4 ℃,the critical temperature that causes chilling injury on most cultivated bananas).The phenotypic differences between Sanming wild banana and‘Tianbaojiao’banana before and after low temperature treatment were measured using the LemnaTec(Germany)plant phenotypic imaging system.Moreover,by using M-series modulated chlorophyll fluorescence imaging system IMAGING-PAM(Germany,Walz),the chlorophyll fluorescence parameters on the second leaf of the seedlings prior to and 24 h after low temperature treatment were measured.The measured indexes included the maximal photochemistry ef
作者 李丹 黄玉吉 朱宁 刘范 田娜 徐涵 程春振 赖钟雄 LI Dan;HUANG Yuji;ZHU Ning;LIU Fan;TIAN Na;XU Han;CHENG Chunzhen;LAI Zhongxiong(lnstitute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Institut de la Recherche lnterdisciplinaire de Toulouse,Toulouse 31300,France)
出处 《果树学报》 CAS CSCD 北大核心 2019年第1期76-85,共10页 Journal of Fruit Science
基金 国家自然科学基金(31601713) 国家香蕉产业体系专项资金(CARS-32-11) 福建省中青年教师教育科研项目(JA15189,JAT160166) 福建农林大学“校杰出青年科研人才”计划项目(xjq201721).
关键词 三明野生蕉 细胞结构紧密度 叶绿素荧光 低温胁迫 抗氧化酶活性 Sanming wild banana (Musa itinerans) Cell tense ratio Chlorophyll fluorescence Cold stress Antioxidant enzyme activity
作者简介 李丹,女,在读硕士研究生,主要从事园艺植物逆境生理与分子生物学研究。Tel:13305929789,E-mail:1003411141@qq.com;通信作者:程春振E-mail:ld0532cheng@126.com;通信作者:赖钟雄E-mail:Laizx01@163.com.
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