目的观察莫诺苷对脑缺血再灌注大鼠细胞周期相关蛋白表达的影响。方法 Sprague-Dawley大鼠用线栓法制备大脑中动脉栓塞(middle cerebral artery occlusion,MCAO)再灌注模型后,随机分为假手术组、模型组、莫诺苷小剂量组(30 mg/kg体质量)、莫诺苷中剂量组(90 mg/kg体质量)、莫诺苷大剂量组(270 mg/kg体质量)。造模7 d后,用Western blot方法检测大鼠患侧海马细胞周期相关蛋白CyclinD1及CDK6的表达情况。结果与假手术组相比,模型组CyclinD1蛋白表达显著增加;与模型组相比,莫诺苷中剂量组与大剂量组CyclinD1显著降低。与假手术组相比,模型组CDK6蛋白表达显著增加;与模型组相比,莫诺苷小、中、大三个剂量组CDK6蛋白表达显著降低。结论莫诺苷能降低大鼠脑缺血再灌注后CyclinD1和CDK6的表达,从而抑制细胞周期相关蛋白的异常激活,发挥神经保护作用。
Objective To explore the effects of morroniside on cell cycle-related proteins in a rat model of focal cerebral ischemia-reperfusion. Method After the model of middle cerebral artery occlusion(MCAO) was established, Sprague-Dawley rats were randomly divided into sham group, model group, morroniside low dose(30 mg/kg) group, morroniside middle dose(90 mg/kg) group and morroniside high dose(270 mg/kg) group. Western blot analysis was used to measure the expression of CyclinD1 and Cdk6 in ischemic ipsilateral hippocampus. Result Compared with sham group, the expression of CyclinD1 was increased obviously in vehicle-treated ischemic-reperfusion rat. Compared with model group, the expression of CyclinD1 was significantly reduced by treatment of morroniside at dose of 90 mg/kg and 270 mg/kg after ischemia-reperfusion. Compared with sham group, the expression of CDK6 was increased obviously in vehicle-treated ischemic-reperfusion rat. Compared with model group, the expression of CDK6 was significantly reduced by treatment of morroniside at dose of 90 mg/kg and 270 mg/kg after ischemia-reperfusion. Conclusion Morroniside could inhibit the inappropriate activation of cell cycle-related proteins and play neuroprotective effects by downregulating the expression of CyclinD1 and Cdk6 after cerebral ischemia-reperfusion.
Shiyan Dongwu Kexue