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金黄色葡萄球菌P83中插入片段ISSA1的特征与分布 预览

Characterization and distribution of insertion sequence ISSA1 in Staphylococcus aureus P83
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摘要 目的:研究金黄色葡萄球菌P83中插入片段ISSA1的特征与分布.方法:根据曼尼阿蒂斯方法制备金黄色葡萄球菌P83的染色体DNA,利用核苷酸序列分析鉴定ISSA1,并通过Southern印迹法对其检测.结果:ISSA1是属于IS30家族的一个新的插入元件,并广泛存在于葡萄球菌中.结论:在细菌中ISSA1能够水平转移. Objective To study the characterization and distribution of insertion sequence IS SA 1 in Staphylococcus aureus P83.Methods Chromosome DNA was prepared from S.aureus P83 according to the molecular cloning method described by Maniatis.IS SA 1 was identified by nucleotide sequence analysis and detected by Southern blotting analysis.Results IS SA 1 is a new insertion sequence that belongs to a member of the IS30 family,and widely distributed in staphylococcal species.Conclusion The IS SA 1 could transfer horizontally among the different bacteria.
作者 邹丹 金子淳 神尾好是 ZOU Dan 1,Jun KANEKO 2,Yoshiyuki KAMIO 2 (1.Department of Pathogenic Microbiology and Immunology,School of Basic Medical Science,Southeast University,Nanjing 210009,China;?2.Department of Applied Microbiology,Graduate School of Agricultural Science,To
出处 《东南大学学报:医学版》 CAS 2002年第1期 10-15,共6页 Journal of Southeast Univ: Medical Sci Ed
基金 日本文部省基金及上原纪念财团联合资助项目,东南大学校科研和教改项目
关键词 PantonValentineleukocidin 插入片段 原噬菌体 反向重复 直接重复 Panton Valentine leukocidin insertion sequence prophage inverted repeat direct repeat
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  • 1[1]KANEKO J,MURAMOTO K,KAMIO Y.Gene of the LukF-PV-Like component of Panton-Valentine leukocidin in Staphylococcus aureus P83 is linked with lukM[J]. Biosci Biotechnol Biochem, 1997,61:541-544. 被引量:1
  • 2[2]HACKER J,BLUM-OEHLER G,MUHLAORFER I,et al.Pa~thogenicity islands of virulent bacteria: structure, function and impact on microbial evolution[J].Mol Microbiol, 1997,23:1089-1097. 被引量:1
  • 3[3]MANIATIS T,FRITSCH E F,SAMBROOK J.Molecular cloning:A laboratory manual[M].2nd ed,New York:Cold Spring Harbor Laboratory, Cold Spring Harbor,1989. 被引量:1
  • 4[4]BATEMAN A,BIRNEY E,DURBIN R,et al.Pfam 3.1:1313 multiple alignments match the majority of proteins[J].Nucleic Acids Res,1999,27:260-262. 被引量:1
  • 5[5]LEE C Y,IANDOLO J J.Structural analysis of staphylococcal bacteriophage Φ11 attachment sites[J].J Bacteriol,1988,170:2409-2411. 被引量:1
  • 6[6]GALAS D J,CHANDLER M.Bacterial insertion sequences[A].In:Berg D,Howe M M.Mobile DNA[M].Washington:American Society for Microbiology,1989.109-162. 被引量:1
  • 7[7]MAHILLON J,CHANDLER M.Insertion sequences[J].Microbiol Mol Biol Rev,1998,62:725-774. 被引量:1
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