The genetic polymorphism and molecular phylogeny of 24 species and varieties in section Thea genus Camellia were investigated. High quality and yield sample DNAs were isolated from winter shoots, the average A260/280 was 1.71 and the average yield was 331μg/g fresh base. Fifteen decamer oligonucleotide primers were selected from the 61 screened. A total of 102 polymorphic bands (6.8 polymorphisms/primer) out of 107 reproducible products (7.1 fragments/primer) were amplified from the selected 15 primers, corresponding to 95.3% polymorphism of the amplification bands. The relative polymorphic frequency ranged from 0.04 to 0.96. However, the general relative frequency of polymorphic was low with an average of 0.30, varying from 0.16 to 0.60. Molecular phylogenetic dendrogram of section Thea was constructed using UPGMA and based on Nei and Lis similarity coefficient from pairwise comparisons of RAPD data between 24 species and varieties. Principal coordinate analysis using SHMM method was also conducted. The species and varieties of section Thea could be divided into two groups, 5-locule ovary group and 3-locule ovary, and further be divided into 3 and 2 subgroups, respectively. The genetic relationship and molecular evolution tendency of some species were discussed from the genetic distance.
Journal of Tea Science