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Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on Cholecystokinin Receptor 1-Mediated Signal Transduction of Pancreatic Acinar Cells in Acute Necrotizing Pancreatitis Rats

Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on Cholecystokinin Receptor 1-Mediated Signal Transduction of Pancreatic Acinar Cells in Acute Necrotizing Pancreatitis Rats
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摘要 调查 Chaiqin Chengqi 煎的效果(, CQCQD )(CCKR1 ) 在 cholecystokinin 上,受体 1 调停了在有尖锐坏死的胰腺炎(ANP ) 的老鼠的胰腺的 acinar 房间的信号 transduction。 Objective: To investigate the effect of Chaiqin Chengqi Decoction (柴芩承气汤,CQCQD) on cholecystokinin receptor 1 (CCKR1)-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis (ANP). Methods: Twenty-seven Sprague-Dawley rats were randomized into three groups: the control group, the ANP group, and the CQCQD group (9 in each group). ANP rats were induced by two intraperitoneal injections of 8% L-arginine (pH=7.0, 4.4 g/kg) over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD (CQCQD group) or physiological saline (control and ANP groups) at 2 h interval. And 6 h after induction, pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression, phospholipase C (PLC) and inositol-1,4,5-triphosphate (IP3), and determination of fluorescence intensity (FI) as a measure of intracellular calcium ion concentration [Ca2+]i. Results: The pancreatic histopathological score (6.2± 1.1) and the levels of PLC (1,187.2±228.2μg/mL) and IP3 (872.2±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control (2.8± 0.4, 682.5± 121.8 μ g/mL, 518.4 ± 115.8 μ g/mL) and the CQCQD (3.8± 0.8, 905.3± 78.5 μ g/mL, 611.0±42.5μ g/mL) groups (P〈0.05). [Ca2+]i FI for the ANP group (34.8± 27.0) was higher than that in the control (5.1 ± 2.2) and CQCQD (12.6± 2.5) groups (P〈0.05). The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated (expression ratio=1.761; ,0=0.024) compared with the control group. The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated (expression ratio=0.311; P=0.035) compared with the ANP group. The ratio of gray values of the CCKR1 and β-actin in the ANP group (1.43±0.17) was higher than those in the control (0.70 ± 0.15) and CQCQD (0.79± 0.1
作者 郭佳 金涛 林子琦 王晓翔 杨晓楠 夏庆 薛平 GUO Jia ,JIN Tao ,LIN Zi-qi, WANG Xiao-xiang , YANG Xiao-nan ,XIA Qing , XUE Ping (Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University, Chengdu (610041), China )
出处 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2015年第1期29-35,共7页 Chinese Journal of Integrative Medicine
基金 Supported by the National Natural Science Foundation of China (No. 81072910), and the Science and Technology Supported Program of Sichuan Province, China (No. 2010SZ0068 and 2011SZ0291)
关键词 急性坏死性胰腺炎 胰腺腺泡细胞 胆囊收缩素 信号转导 承气汤 SPRAGUE-DAWLEY 大鼠 介导 acute pancreatitis, Chaiqin Chengqi Decoction, cholecystokinin receptor 1, Chinese medicine
作者简介 Correspondence to: Prof. XUE Ping, Tel: 86-28-85423373, Fax: 86-28-85423373, E-mail: duburongcheng @ 163.com
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