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猪血中白细胞抗菌肽的提取及体外抑菌研究 被引量:2

Studies on the purification and in vitro antibacterial test of the antibacterial peptides from the leukocytes of swine blood
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摘要 为了研究猪血液白细胞抗菌肽对大肠杆菌、鸡源沙门杆菌等的体外抑菌活性,试验采用乙酸萃取、Superdex 30PG凝胶层析纯化,收集组分并检测抑菌活性。结果表明:猪血液中抗菌肽对大肠杆菌、鸡源沙门杆菌均有良好的抑菌活性。说明该方法可用于猪血白细胞抗菌肽的提取并可抑制上述致病菌。 To verify the in vitro antibacterial activity of antibacterial peptides from swine blood leukocytes to Escherichia coli and chicken Salmo- nella from chicken, the acetic acid extraction and gel filtration chromatography with Superdex 30 PG column were used to collect the fractions and test the antibacterial activity. The results showed that the antibacterial peptides in swine blood had good inhibitory activity against Escherich- ia coli and Salmonella from chicken. The result indicates that the method can be used to extract the antimicrobial peptides from the leukocytes of swine blood, and can inhibit these pathogens.
作者 马辉 王永芬 郭宏伟 王静 MA Hui, WANG Yong - fen , GUO Hong - wei, WANG Jing (Department Of Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450011, China)
出处 《黑龙江畜牧兽医》 CAS 北大核心 2015年第2期34-36,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 河南省教育厅2009年度自然科学研究计划项目(2009A230008)
关键词 血液 白细胞 抗菌肽 提取 抑菌 swine blood leukocyte antibacterial peptide extraction antibacterial
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