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烟草青枯菌FQY_4基因组中原噬菌体生物信息学分析 预览 被引量:3

Bioinformatic analysis of prophage in genome of tobacco pathogen Ralstonia solanacearum FQY_4
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摘要 为了解青枯菌(ralstonia solanacearum)基因组中原噬菌体和噬菌体元件的信息,在烟草青枯菌FQY_4全基因组序列的基础上,分析了FQY_4染色体上的原噬菌体,结果显示FQY_4染色体上有1个具有完整噬菌体特征的原噬菌体,3个噬菌体元件。比较青枯菌GMI1000和FQY_4中的原噬菌体的序列信息,结果显示它们具有一定的相似性,其中青枯菌FQY_4的原噬菌体-2与噬菌体φRSA1的相似度为82%,它们有40个编码基因高度同源,相似度为99%以上。这些信息可为青枯菌原噬菌体的多样性分析,噬菌体和青枯菌的协同进化研究奠定基础。 In order to know prophage and its elements in the genome of ralstonia solanacearum, prophages in the chromosome of FQY-4 was analyzed on the basis of its complete genomic sequence. Results showed that there was one prophage which contained the typical characteristics of phage, and three incomplete prophage elements. Compared with the Ralstonia Solanacearum strain GMI1000, FQY_4 shared similarity in prophage, and homology between the prophage-2 in FQY_4 and prophage ?RSA1 was 82%and there were 40 coding sequences with highly significant homology level of more than 99%, which laid solid foundation for diversity analysis of prophage of Ralstonia Solanacearum, and for co-evolution of Ralstonia Solanacearum and phages.
作者 蔡刘体 刘艳霞 孟琳 罗正友 石俊雄 CAI Liuti, LIU Yanxia, MENG Lin, LUO Zhengyou, SHI Junxiong (Guizhou Tobacco Research Institute, Guiyang, 550081,China)
出处 《中国烟草学报》 EI CAS CSCD 北大核心 2015年第1期82-88,共7页 Acta Tabacaria Sinica
基金 中国烟草总公司贵州省公司科技项目“烟草青枯菌致病基因家族分析”(合同号:黔烟科合2012-03) 农业部公益性行业(农业)科研专项项目“利用有机(类)肥料调控我国土壤微生物区系关键技术研究”(合同号:201103004)
关键词 烟草 青枯病 青枯菌 基因组 原噬菌体 tobacco bacterial wilt Ralstonia solanacearum genome prophage
作者简介 蔡刘体(1974-),博士,副研究员,从事土肥植保工作,Email:cailiuti01@163.com 通讯作者:石俊雄(1966-),研究员,主要从事烟草营养与施肥,植烟土壤微生物生态修复工作,Email:sjx2196@163.com
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