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马铃薯青枯病病原的鉴定 预览 被引量:2

Identification of Potato Bacterial Wilt Pathogens
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摘要 利用湖北省四个地点的马铃薯青枯病样本,对其病原物的菌落形态、碳水化合物利用、显微形态、鞭毛引物序列、演化型以及致病力等方面进行了分析。结果显示,在2,3,5-氯化三苯基四氮唑培养基上检测得到16个病原物菌落符合青枯菌菌落形态特征,包括菌落圆形,隆起,中间红色,乳白色分泌物,并伴有褐色物质;显微观察16个病原物中仅有6个病原物具有短杆状、两端钝圆的特点;而其中能够通过PCR扩增得到鞭毛序列的病原物仅有源自武汉的HZ4-14和HZ5-1,序列比对发现两者序列一致且与GMI1000等已测序青枯菌株同源性达到99%;演化型测定结果显示HZ4-14和HZ5-1均能得到144 bp的目标条带,为演化II型;利用四种不同马铃薯材料试管苗伤根接种鉴定结果显示两个菌株均具有一定致病力。结果表明,引起湖北省武汉市青枯病的病原物为雷尔氏青枯菌Ralstonia solanacearum,生理小种2号,演化II型(美洲组)。 The colony morphology, carbohydrate utilization, microscopy, fliC sequence, phylotype and pathogenicity of Ralstonia solanacearum were studied by using the diseased potato from four cities of Hubei Province in order to investigate the etiology of the disease bacterial wilt. Sixteen strains exhibited irregular round, upheaval, pink colonies with white margin differential y on BG medium with 2,3,5-triphenyl tetrazolium chloride. Six strains of them showed the targeted morphology of R. solanacearum by microscope. Only two strains had targeted fragments, which were amplified by the specific fliC primers. The targeted fragments were sequenced. The results revealed that HZ4-14 and HZ5-1 had 99%nucleotide matching with the fliC gene of R. solanacearum GMI1000. The results of phylotype multiple PCR indicated that the specific 144 bp fragments were amplified from two strains. HZ4-14 and HZ5-1 were virulent to potato and showed the typical bacterial wilt symptom. These results demonstrate that the pathogen of bacterial wilt in Wuhan belongs to Bivoar II, Phylotype II of R. solanacearum.
作者 郑雪坳 宋波涛 谭晓丹 陈惠兰 ZHENG Xueao, SONG Botao, TAN Xiaodan, CHEN Huilan ( College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology (HAU), Ministry of Education/National Center for Vegetable Improvement (Central China), Huazhong Agricultural University,Wuhan, Hubei 430070, China )
出处 《中国马铃薯》 2014年第2期83-89,共7页 Chinese Potato
基金 华中农业大学自主科技创新基金项目“转录组结合快速基因定位策略解析马铃薯青枯病抗性的调控网络”(2013PY080) 新进高层次人才科研启动基金项目“马铃薯抗青枯病基因的定位和挖掘”(201209)
关键词 马铃薯 青枯菌 病原鉴定 potato Ralstonia solanacearum identification
作者简介 郑雪坳(1989一),男,硕士研究生,研究方向为植物抗病生物学。 通信作者:陈惠兰,教授,从事植物逆境生物学和遗传育种方面的研究,E-mail:chenhuilan@mail.hzau.edu.cn。
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