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六味地黄丸对肾阴虚大鼠肺肾组织水通道蛋白1表达的影响 被引量:15

Effects of Liuwei Dihuang Wan on AQP1 expression of lung and kidney in kidney-yin deficiency model rats
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摘要 目的:观察六味地黄丸对肾阴虚大鼠肺、肾组织水通道蛋白1(AQP1)表达的影响。方法:将40只SD大鼠随机分为对照组(10只)、甲状腺素组(30只),采用甲状腺素混悬液灌胃复制肾阴虚大鼠模型4周,造模成功后将模型组大鼠随机分为3组:甲状腺素组、六味地黄丸低剂量组和六味地黄丸高剂量组,每组10只,给药组给予相应剂量六味地黄丸混悬液灌胃4周,观察记录各组大鼠体质量、饮水量和尿量等一般症状体征;末次给药后,实时荧光定量PCR检测大鼠肺、肾组织中AQP1 mRNA的表达,Western Blot测定肺、肾组织AQP1蛋白的表达。结果:与对照组比较,甲状腺素组大鼠体质量显著降低(P〈0.01),饮水量明显增多(P〈0.01),尿量显著减少(P〈0.01);肺、肾组织中AQP1 mRNA和蛋白表达显著高于对照组(P〈0.01);给予六味地黄丸干预后肾阴虚大鼠的一般症状体征得到显著改善,肺、肾组织中AQP1 mRNA和蛋白的表达显著降低(P〈0.01)。结论:六味地黄丸可有效纠正肾阴虚大鼠水液代谢紊乱,其机制可能与下调肺、肾组织中AQP1 mRNA和蛋白表达有关。 Objective: To observe the intervention effects of Liuwei Dihuang Wan(LW) on the expression of AQP1 in lung and kidney in kidney-yin deficiency rats.Methods: forty SD rats were randomly divided into: normal control group,thyroxine group(n=10) and thyroxine group(n=30); Model of kidney yin deficiency rats was produced by ig administrating tetraiodothyronine per day.Four weeks after this procedure,rats in thyroxine group were randomly classified into thyroxine group,and two treatment groups(high dose group and low dose group); treatment groups were ig administered once daily for 4 weeks.Observing records of each rats' body weight,water quantity and general symptoms and signs such as urine; After the last delivery,AQP1 mRNA was assayed by real time PCR.The protein was measured by Western Blot.Results: Compared with normal control group,the thyroxine rats had a low level of body weight,water intake and low level of urinary output(P〈0.01),AQP1 protein and mRNA(P〈0.01).Compared with the model rats,the protein levels of AQP1 and mRNA expression of AQP1 in lung and kidney were all significantly reduced after treatment with low or high dose of LW-medicated(P〈0.01).Conclusion: Liuwei Dihuang Wan can reduce AQP1 level in kindey-yin deficiency model rats.It is suggested that may be one of the fluid metabolism regulation mechanisms of Liuwei Dihuang Wan in kindey-yin deficiency.
作者 徐文聃 戴世杰 李哲明 朱延涛 陈宜涛 李昌煜 XU Wen-dan, DAI Shi-jie, LI Zhe-ming, ZHU Yan-tao, CHEN Yi-tao, LI Chang-yu (1Zhejiang Chinese Medical University, Hangzhou 310053, China; 2Jinhua Hospital of TCM, Jinhua 321017, China )
出处 《中华中医药杂志》 CAS CSCD 北大核心 2015年第4期1242-1245,共4页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 国家自然科学基金项目(No.81173143,No.81373507)
关键词 六味地黄丸 肾阴虚 水通道蛋白1 甲状腺素 Liuwei Dihuang Wan Kindey-yin deficiency Aquaporin 1 Thyroxine
作者简介 通讯作者:李昌煜,浙江省杭州市滨江区滨文路548号浙江中医药大学,邮编:310053,电话:0571—86613537,E—mail:lml59@sina.Com
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