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神经肽Y对小神经胶质细胞活化状态和生成TNF-α的影响及其作用机制 预览 被引量:3

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摘要 目的:探讨神经肽 Y(neuropeptide Y,NPY)对原代小神经胶质细胞生物活性及生成肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的影响及其作用机制。方法培养原代大鼠皮层小胶质细胞,将细胞分为 Control组、脂多糖(lipopolysaccharide,LPS)组、NPY+LPS 组、NPY 组和 BIBP3226+NPY+LPS 组,分别用无血清胶质细胞培养液、含 LPS 的无血清胶质细胞培养液、含 NPY 和 LPS 的无血清胶质细胞培养液、含 NPY 的无血清胶质细胞培养液和含 BIBP3226、NPY 和 LPS 无血清胶质细胞培养孵育细胞6 h。经免疫细胞化学荧光染色后,显微镜下观察小胶质细胞的形态学变化。ELISA 方法检测培养液中 TNF-α蛋白含量,RT-PCR 方法检测小胶质细胞中TNF-αmRNA 表达水平。结果 LPS 组 TNF-α的含量及细胞中 TNF-αmRNA 表达水平显著高于 Control 组(P <0.05),小胶质细胞处于活化状态;LPS+ NPY 组与 LPS 组相比,TNF-α蛋白和 mRNA 表达水平明显降低(P <0.05),小胶质细胞活化水平降低;BIBP3226+NPY+LPS 组与 LPS+NPY 组相比,TNF-α蛋白和 mRNA 表达水平显著增高(P <0.05);LPS 组与 BIBP3226+NPY+LPS 组差异无统计学意义;NPY 组与 Control 组差异无统计学意义。结论 NPY 可以降低小神经胶质细胞的生物活性,抑制其生成 TNF-α,作用机制可能与 NPY Y1受体有关。
出处 《河北医科大学学报》 CAS 2015年第4期463-466,共4页 Journal of Hebei Medical University
作者简介 李琦军(1972-),男,河北正定人,河北省石家庄市第三医院副主任医师,医学博士,从事创伤骨科疾病诊治研究。 通讯作者
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