期刊文献+

F10基因过表达及沉默对绒癌细胞系JEG-3侵袭性的影响 被引量:1

Influence of F10 gene depletion or over-expression on the invasiveness of choriocarcinoma cell line JEG-3
分享 导出
摘要 目的探讨F10基因过表达及沉默对绒癌细胞系JEG-3侵袭相关蛋白酶表达的影响。方法采用细胞转染技术及RNA干扰技术,分别建立和筛选出F10基因稳定过表达及沉默的绒癌细胞系JEG-3。将SPF级裸鼠(4~5周龄)30只随机分为F10过表达组、F10沉默组和未处理组各10只,分别接种F10基因过表达的JEG-3细胞、F10基因沉默的JEG-3细胞和未处理的JEG-3细胞。于接种5周后处死裸鼠取皮下肿瘤,采用免疫组织化学法和Western blot法检测各组瘤体组织中基质金属蛋白酶(matrix metalloproteinase,MMP)、基质金属蛋白酶组织抑制物-1(tissue inhibitor of matrix metalloproteinase,TIMP-1),血浆纤溶酶原激活物抑制因子(plasminogen activator inhibitor-1,PAI-1)等侵袭相关蛋白酶的表达,并进行比较。结果免疫组织化学结果显示,F10过表达组成瘤组织中MMP-2、MMP-8、MMP-11、MMP-16、MMP-19蛋白表达水平(0.332±0.037、0.473±0.089、0.441±0.031、0.470±0.040、0.548±0.062)高于未处理组(0.209±0.021、0.314±0.108、0.207±0.025、0.400±0.057、0.466±0.038)和F10沉默组(0.049±0.013、0.168±0.034、0.149±0.024、0.240±0.024、0.175±0.029)(P〈0.05),未处理组高于F10沉默组(P〈0.05);F10过表达组TIMP-1(0.191±0.027)和PAI-1蛋白(0.130±0.020)表达水平低于未处理组(0.249±0.036、0.284±0.020)和F10沉默组(0.310±0.018、0.463±0.115)(P〈0.05),未处理组低于F10沉默组(P〈0.05)。wesrern blot检测结果显示,上述侵袭相关蛋白酶在3组间比较差异均有统计学意义(P〈0.05)。结论 F10通过上调MMP-2、MMP-8、MMP-11、MMP-16、MMP-19表达,下调TIMP-1、PAI-1表达,促进绒癌细胞增殖、侵袭和转移。 Objective To investigate the influence of F10 gene depletion or over-expression on the expression of invasionrelated proteinase in choriocarcinoma cell line JEG-3.Methods Stable F10 gene over-expression or F10gene-silenced JEG-3choriocarcinoma cell lines were established by cell transfection and RNA interference technology.Thirty SPF nude mice aged from 4to 5 weeks were randomly divided into F10over-expression group(by injecting of F10 gene overexpressed JEG-3cells),F10gene-silenced group(by injecting of F10gene-silenced JEG-3cells)and control group(by injecting of non-treated JEG-3cells),with 10 mice in each group.The mice were sacrificed and the subcutaneous tumor tissues were gained 5weeks after the JEG-3cell injection.Western blot and immunohistochemical method were used to detect the expressions of matrix metalloproteinase(MMP)-2,MMP-8,MMP-11,MMP-16,MMP-19,tissue inhibitors of metalloproteinase-1(TIMP-1),and plasminogen activator inhibitor-1(PAI-1)in subcutaneous tumor tissues in three groups.Results The immunohistochemical method revealed the expressions of MMP-2,MMP-8,MMP-11,MMP-16 and MMP-19 were the highest in F10over-expression group(0.332±0.037,0.473±0.089,0.441±0.031,0.470±0.040,0.548±0.062),followed by control group(0.209±0.021,0.314±0.108,0.207±0.025,0.400±0.057,0.466±0.038)and F10 silenced group(0.049±0.013,0.168±0.034,0.149±0.024,0.240±0.024,0.175±0.029)in turns(P0.05).The expressions of TIMP-1and PAI-1 were the lowest in F10over-expression group(0.191±0.027,0.130±0.020),followed by control group(0.249±0.036,0.284±0.020)and F10 silenced group(0.310±0.018,0.463±0.115)in turns(P0.05).Western blot showed significant differences in the above invasion-related proteinase indexes among three groups(P0.05).Conclusion F10 gene could up-regulate the expressions of MMP-2,MMP-8,MMP-11,MMP-16 and MMP-19,and down-regulate the expressions of TIMP-1and PAI-1,which is involved in the proliferation,invasion and metastasis of choriocarc
作者 苏晓华 庞战军 SU Xiao Hua, PANG Zhan-Jun (Department of Obstetrics and Gynecology, Nan fang Hospital of Southern Medical University, Guangzhou 510515, China)
出处 《中华实用诊断与治疗杂志》 2015年第5期444-447,共4页 Journal of Practical Diagnosis and Therapy
基金 国家自然科学基金(81170595)
关键词 绒癌 F10基因 基质金属蛋白酶 血浆纤溶醇原激活物抑制因子 JEG-3细胞 Choriocarcinoma F10gene matrix metalloproteinase plasminogen activator inhibitor JEG-3cell
作者简介 通信作者:庞战军,E—mail:zjpang@yeah.net。
  • 相关文献

参考文献13

二级参考文献80

  • 1周瑾 ,陈士岭 ,邢福祺 ,庞战军 ,李冰 ,周问渠 ,付欣 ,丁彦青 .葡萄胎发病新基因F10与滋养细胞肿瘤侵袭相关性研究[J].第一军医大学学报,2005,25(2):171-173. 被引量:17
  • 2周瑾 ,梁卫华 ,李冰 ,陈士岭 ,邢福祺 ,庞战军 ,周问渠 ,付欣 ,丁彦青 .葡萄胎发病新基因F10在不同肿瘤组织的表达[J].广东医学,2005,26(5):596-597. 被引量:15
  • 3周瑾,邢福祺,余良宽,庞战军,冯桂萍.葡萄胎发病新基因F10在原发性肝癌中的表达分析[J].解放军医学杂志,2007,32(6):601-603. 被引量:6
  • 4Whitley G S, Dash P R, Ayling I. J, et al. Increased apoptosis in first trimester extravillous trophohlasts from pregnancies at higher risk of developing preeclampsia[J]. Am J Pathol, 2007. 170(6) :1903- 1909. 被引量:1
  • 5Cohen M, Bischof P. Factors regulating trophoblast invasion [J]. Gynecol Obstet Invest, 2007,64(3):126 -130. 被引量:1
  • 6Bischof P, Meisser A, Campana A. Paracrine and autocrine regulators of trophoblast invasion: a review[J]. Placenta, 2000, 21 Suppl A (14):S55-S60. 被引量:1
  • 7Meshche Hakova LA,Kozachenko VP.Role of surgicall interventions in the treatment of patients with trophoblastic tumor resistant to chem[J].Otherapy Vopr Onkol,1998,44(5):610-614. 被引量:1
  • 8Suzuka K,Matsui H,Iitsuka Y,et al.Adjuvant hysterectomy in low-risk gestational trophoblastic sisease[J].Obstest Gynecol,2002,97(3):431-434. 被引量:1
  • 9Jonathan SB,Neville FH.实用妇科肿瘤学[M].南京:江苏科学技术出版社,2005:490. 被引量:1
  • 10宋鸿钊.滋养细胞肿瘤的诊断与治疗[M].北京:人民卫生出版社,1983.168. 被引量:5

共引文献28

同被引文献16

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈