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固定化青霉素酰化酶合成阿莫西林活性测定 预览

Measurement of Activity of Synthesis Amoxicillin from Immobilized Penicillin Acylase
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摘要 固定化青霉素酰化酶的活性高低是影响酶催化合成阿莫西林速度的决定性因素,如何测定固定化青霉素酰化酶的合成活性起着至关重要的作用。目前,对青霉素酰化酶活性的测定主要集中在青霉素酰化酶的水解活性,而对其合成阿莫西林活性的研究较少,这导致不能快速的鉴别青霉素酰化酶合成阿莫西林的快慢,不能有效的指导生产。文章对固定化青霉素酰化酶合成阿莫西林的活性进行了定义,并研究了合成活性的测定方法,该测定方法准确可靠,操作简捷,便于比较不同厂家生产的青霉素酰化酶活性的高低,对工业生产起指导作用。 The level of activity of immobilized penicillin acylase is the important factor which affects the speed of enzymatic synthesis of amoxicillin, thus it plays signiifcant role how to determine synthetic activity of immobilized penicillin acylase. Currently, the determination of activity of penicillin acylase is focus on hydrolytic activity of penicillin acylase. But there is lack of the reports on determination in activity of synthesis of amoxicillin, which leads to the fact that fast determination of the synthesis speed from penicillin acylase to amoxicillin may be impossible and the production cannot be guided from theory. In this article, the activity of amoxicillin synthesized from penicillin acylase was deifned and the method for measuring the activity was studied. It was shown that this method is easy to operate and the result is accurate and reliable. Moreover, by using this method the activities of penicillin acylases produced from different factories are easy to be compared so that the comparing results can be used as guidance for production.
作者 严正人 朱军 刘慧勤 魏士倩 张海燕 李红颖 刘丹 Yan Zhengren,Zhu Jun,Liu Huiqing,Wei Shiqian,Zhang Haiyan,Li Hongying,Liu Dan(NCPC Xian Tai Pharmaceutical Co., Ltd, Shijiazhuang, 052165)
出处 《化工与医药工程》 2015年第1期23-25,共3页 Pharmaceutical Engineering Design
关键词 固定化 青霉素酰化酶 阿莫西林 合成活性 immobilization penicillin acylase amoxicillin synthesis activity
作者简介 严正人(1965-),男,高级工程师,主要从事科研开发及管理工作。
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