The Hypocotyls of a new industrial hemp cuhivar Long - ma No. 1 were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on. The best combination of plant growth regulators was 1.0 mg/L 6 - BA and 0. 5 mg/L NAA for the induetion of callus. The best combination of hormones was 1.0 mg/L KT and 0. 5 mg/L NAA for the differentiation rate of adventitious bud. The best combination was 1/2 MS ＋ 0. 05 mg/L IBA ＋ 0. 05 mg/L NAA for rooting. The efficient regeneration system for industrial hemp was built preliminarily. The induction rate of callus was 91.67%, the differentiation rate of adventitious bud was 76. 67%, the induction rate of root was 75 %.
Plant Fibers and Products
industrial hemp （Cannabis sativa L ）