期刊文献+

海南分离出一株产新德里金属β-内酰胺酶-1基因的产气肠杆菌 预览

Separation for one strains New Delhi metallo-beta-lactamas-1 gene of Enterobacter aerogene in isolated Hainan of China
在线阅读 下载PDF
分享 导出
摘要 目的分离与鉴定海南地区临床发现的耐碳青酶烯类药物的产气肠杆菌是否携带新德里金属β-内酰胺酶-1(New Delhi metallo-beta-lactamas-1,NDM-1)基因。方法用法国梅里埃API 20E生化鉴定条和VITEK2全自动细菌鉴定仪及其配套CNS试剂,鉴定出临床耐亚胺培南和美洛培南的产气肠杆菌,并用亚胺培南/亚胺培南+EDTA纸片法协同试验测定产金属酶类碳青霉烯酶;然后,对目的菌株进行NDM-1耐药基因聚合酶链反应(polymerase chain reaction,PCR)特异性扩增后,将扩增产物行琼脂糖凝胶电泳和测序鉴定及BLAST比对,以确定其NDM-1基因。结果采用亚胺培南/亚胺培南+EDTA纸片法协同试验,从10株耐亚胺培南或美洛培南的产气肠杆菌中,检出4株金属类碳青霉烯酶表型阳性菌株。对其中4株金属酶表型阳性的产气肠杆菌进行NDM-1基因PCR扩增后,对其产物进行琼脂糖凝胶电泳和测序分析,确认1株为携带NDM-1的耐药基因菌株。结论海南发现1株携带bla NDM-1基因的产气肠杆菌。 Objective To isolate and confirm the reduced carbapenem susceptible Enterobacter aerogene with NDM-1 (New Delhi metallo-beta-lactamas-1) in Hainan. Methods The positive Enterobacter aerogene strains, which had the ability to hydrolyzing Imiperem and Meropenem, were discerned by BioMerieux API 20E identification system and automatic VITEK-2 dentification system. The production of metalloprotease carbapenemase was measured synergically using imipenem/imipenem+EDTA paper method. The specific NDM-1 genes was identified by sequencing of products by polymerase chain reaction(PCR) amplification. Results From ten to four positive strains were identified as metalloprotease carbapenemase positive in 10 imipenem or meropenem- resistant Enterobacter aerogene by imipenem/imipenem+EDTA paper method. One strain was confirmed as NDM- 1 positive strain by PCR sequencing. Conclusion One Enterobacter aerogene strain carrying blaNDM-1 gene was identified in Hainan.
作者 李天娇 张强 符生苗 王旭明 黄涛 符惠群 Li Tianjiao, Zhang Qiang, Fu Shengmiao, Wang Xuming, Huang Tao, Fu Huiqun( Departmant of Laboratory, Hainan Hospital, Haikou 570311, China)
出处 《中华临床实验室管理电子杂志》 2015年第2期109-112,共4页 CHINESE JOURNAL OF CLINICAL LABORATORY MANAGEMENT(Electronic Edition)
基金 海南省自然基金资助项目(814307)
关键词 耐碳青酶烯类抗生素 新德里金属β-内酰胺酶-1基因 产气肠杆菌 Resistance of Carbapenems drugs New Delhi metallo-beta lactamas 1 Multiresistan Aerogenes
作者简介 通讯作者:符生苗,Email:smfu2000@126.com
  • 相关文献

参考文献10

  • 1Kumarasamy KK, Toleman MA, Walsh TR, et al. Emergenceof a new ant ibiot ic resist an ee m echani sm in India, Pakistan, and the UK: a molecular, biological, and epidemiologi- calstudy[J]. Lancet Infect Dis, 2010,10(9):597-602. 被引量:1
  • 2卓超,钱元恕.金属β—内酰胺酶的研究进展[J].国外医药:抗生素分册,2003,24(1):12-17. 被引量:19
  • 3Yong D, Toleman MA, Giske CG, et al. Characterization ot a newmetallo-beta-lactamase gene,bla(NDM-1), and a novel erythnmyein esteraae gene carried on a unique genetic structure in Klebsiella Uapneumoniae sequence type 14 from India[J]. Antimicrob Agents Chemother, 2009,53(12):5046-5054. 被引量:1
  • 4黄留玉.关于超级细菌NDM-1的若干思考[J].解放军医学杂志,2010(12):1409-1411. 被引量:17
  • 5中华人民共和国卫生部医政司.关于做好泛耐药肠杆菌科细菌检测工作的通知.2010. 被引量:1
  • 6中华人民共和国卫生部.产NDM.1泛耐药肠杆菌科细菌感染诊疗指南(试行版).2010. 被引量:1
  • 7Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: M100-S20-U.CLSI[S]. Wayne: PA, USA, CLSI, 2010. 被引量:1
  • 8Yong D, Lee K, Yum JH, et al. Imipenem-EDTA disk method for differentiation of metallo-beta-laetamase-producing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J Clin Microb, 2002,40(10):3798-3801. 被引量:1
  • 9郝琼,刘翔,郭邦成,谢明英,闫立群,王鑫,景怀琦.宁夏腹泻病人群携带NDM-1基因菌株的初步研究[J].宁夏医学杂志,2012,34(4):289-291. 被引量:12
  • 10陈硕,邱少富,夏力亮,王中强,刘军,柳楠,祝令伟,孙洋,宋宏彬,冯书章.鲍曼不动杆菌blaNDM-1基因序列分析及表达[J].中国人兽共患病学报,2012,28(5):471-473. 被引量:11

二级参考文献22

  • 1Payne DJ Cramp R Bateson J H et al.Rapid identification of metallo-and serine β-lactamases [J].Antimicrob Agen ts Chemother,1994,38:991-991. 被引量:1
  • 2Kumarasamy-KK, Toleman MA, Walsh TR, et al. Emergence of a new antibiotic resistance mechanism in India, Pakistan and the UK: a molecular, biological, and epidemiological study [J]. Lancet, 2010,8 : 1016 - 1022. 被引量:1
  • 3Yong D, Toleman MA, Giske CG, et al. Characterization of a new metallo - β- lactamase gene, bla NDM - I and a novel erythromycin esterase gene carried on a unique genetic structure in klebsiella pneumoniae sequance type 14 from India[J]. Antimicrobial Agents and Chemotherapy ,2009,10:5046 - 5054. 被引量:1
  • 4Poirel L, Lagrutta E, Taylor P, et al. Emergence of metallo -β- lac- tamase NDM -I -producing muhidrug resistant escherichia coli in Australia. American Society for Microbiology [J]. Antimicrobial Agents and Chemotherapy,2010,10:878 - 884. 被引量:1
  • 5RoseWE,RybakhMJ.Tigecyeline:firstofDewclass0fantimi-crobialagents[J].Phannacotherapy,2006,26(8):1099-1110. 被引量:1
  • 6Yong D, Toleman MA, Giske CG, et al. Characterization of new metallo-beta-lactamase gene, bla(NDM-1), and a novel e- rythromyein esterase gene carried on a unique genetc structure in Klebsiella pneumonia sequence type 14 from India [J]. Antimi- crob Agents Chemother, 2009, 53(12):5046-5054. 被引量:1
  • 7Samuelsen O, Castanheira M, Walsh TR, et al. Kinetic charac-terization of VIM-7, a divergent member of the VIM metallo-be- ta-lactamase family [J], Antimierob Agents Chemother, 2008, 52(8) : 2905-2908. 被引量:1
  • 8Murphy TA, Simm AM, Toleman MA, et al. Biochemical Characterization of the Acquired Metallo-beta Lactamase SPM-1 from Pseudomonas aeruginosa [J]. Ant imicrohial Agents and Chemotherapy, 2003, 47(2): 582-587. 被引量:1
  • 9Lee K, Yum J H, Yong D, et al. Novel acquired metallo-beta- lactamase gene, blaSIM-1, in a class 1 integron from Acineto- bacte r baumannii clinical isolates from Korea[J]. Antimicrob A- gents Chemother, 2005, 49(11): 4485-4491. 被引量:1
  • 10Sobel JD. Pathogenesis and treatment of recurrent vulvovaginal candidiasis[J]. Clin Infect Dis,1992,14(Suppll) :S148-153. 被引量:1

共引文献46

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈