期刊文献+

携带blaKPC-2基因的肺炎克雷伯菌ST789型引起医院感染暴发 被引量:4

Outbreak of nosocomial infections due to Klebsiella pneumoniae ST789 isolates harboring blaKPC-2 gene
分享 导出
摘要 目的探讨医院耐碳青霉烯类抗菌药物肺炎克雷伯菌和大肠埃希菌的分子流行病学,为医院感染的预防和控制提供指导。方法收集医院2013年2-4月分离的对厄他培南耐药11株肺炎克雷伯菌和4株大肠埃希菌;细菌的鉴定和药敏试验使用法国生物梅里埃公司VITEK-2Compact系统;菌株是否产碳青霉烯酶按改良Hodge试验操作,其携带的β-内酰胺类耐药基则通过多重PCR进行检测,并经测序确认基因型;通过多位点序列分型和脉冲场凝胶电泳试验技术对细菌的同源性进行分析。结果分离的11株耐碳青霉烯类肺炎克雷伯菌多位点序列分型,分别是ST789型7株、ST437株型2和ST258型1株;经测序确认,10株携带有blaKPC-2基因;脉冲场凝胶电泳显示,3种带型A、B和C,7株ST789型肺炎克雷伯菌的带型一致;4株耐碳青霉烯类大肠埃希菌中有2株携带blaKPC-2基因,多位点序列分型均是ST131型;每一株菌可携带不同数目的超广谱β-内酰胺(blaSHV、blaTEM-1和blaCTX)基因。结论医院内存在携带blaKPC-2基因肺炎克雷伯菌引起的医院感染暴发流行,要加强消毒隔离,防止进一步播散。 OBJECTIVE To investigate the molecular epidemiology of the clinical carbapenem-resistant Klebsiella pneumoniae and Escherichia colii isolates so as to provide guidance for prevention and control of nosocomial infections.METHODS Totally 4strains of ertapenem-resistant E.coli and 11 strains of ertapenem-resistant K.pneumoniae were isolated from the hospitals from Feb 2013 to Apr 2013,then the bacterial identification and the drug susceptibility testing were carried out by using VITEK-2Compact system of BioMérieux,France,the modified Hodge test was conduced to determine whether the strains produced carbapenemase,theβ-lactam resistance genes in the strains were detected with the use of multiple PCR,and the genotypes were confirmed through sequencing;the homology of the strains was analyzed by means of multilocus sequence typing and pulsed-field gel electrophoresis(PFGE).RESULTS The multilocus sequence typing of the 11 strains of carbapenem-resistant K.pneumoniae showed that there were 7strains of ST789,2strains of ST437,and 1strain of ST258;the sequencing confirmed that 10 strains carried with blaKPC-2gene.The 10 strains of KPC-2-producing K.pneumoniae were categorized into 3PFGE types(A,B and C),and 7strains of K.pneumoniae ST789 were the same type.2of 4carbapenemresistant E.coli isolates harbored the blaKPC-2gene,and the multilocus sequence typing showed both strains were ST131;each strain could carry with different numbers of extended-spectrumβ-lactams genes(blaSHV,blaTEM-1and blaCTX).CONCLUSIONThe K.pneumoniae harboring the blaKPC-2gene may result in the outbreak of nosocomial infection,thus,it is necessary to strengthen the disinfection and isolation so as to further dissemination.
作者 李青 油丽萍 陈爱文 徐茶 赵辉 贾楠 朱元祺 LI Qing,YOU Li-ping,CHEN Ai-wen,XU Cha,ZHAO Hui,JA Nan,ZHU Yuan-qi(Qingdao Municipal Hospital, Qingdao, Shandong 266071, China)
出处 《中华医院感染学杂志》 CAS CSCD 北大核心 2016年第2期251-253,262共4页 Chinese Journal of Nosocomiology
基金 山东省科技攻关基金资助项目(2009GG10002057) 青岛市开发区重点科技发展基金资助项目(2013-1-82)
关键词 肺炎克雷伯菌 基因 blaKPC-2基因 医院感染 暴发 Klebsiella pneumoniae Gene blaKPC-2gene Nosocomial infection Outbreak
作者简介 通信作者:朱元祺,E—mail:zyudot@aliyun.com
  • 相关文献

参考文献7

  • 1Centers for Disease Control and Prevention(CDC).Notes from the field:hospital outbreak of carbapenem-resistant Klebsiella pneumoniae producing New Delhi metallo-betalactamase--Denver,Colorado,2012[J].Morb Mortal Wkly Rep,2013,62(6):108. 被引量:1
  • 2Wei ZQ,Du XX,Yu YS.Plasmid-mediated KPC-2in a Klebsiella pneumonia isolate from China[J].Antimicrob Agents Chemother,2007,51(2):763-765. 被引量:1
  • 3Poirel L,Walsh TR,Cuvillier V,et al.Multiplex PCR for detection of acquired carbapenemase genes[J].Diagn Microbiol Infect Dis,2011,70(1):119-123. 被引量:1
  • 4Woodford N,Fagan EJ,Ellington MJ.Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrumβ-lactamases[J].J Antimicrob Chemother,2006,57(1):154-155. 被引量:1
  • 5Colom K,Perez J,Alonso R,et al.Simple and reliable multiplex PCR assay for detection of blaTEM,blaSHV and blaOXA-1in Enterobacteriaceae[J].FEMS Microbiol Lett,2003,223(2):147-151. 被引量:1
  • 6Yigit H,Queenan AM,Anderson GJ,et al.Novel carbapenem-hydrolyzing beta-lactamase,KPC-1,from a carbapenemresistant strain of Klebsiella pneumoniae[J].Antimicrob Agents Chemother,2001,45(4):1151-1161. 被引量:1
  • 7李静,胡志东.产碳青酶烯酶肺炎克雷伯菌的检测与分析[J].中华医院感染学杂志,2010(15):2324-2326. 被引量:7

二级参考文献10

  • 1Anderson KF, Lonsway DR, Rasheed JK,et al. Evaluation of methods to identify the Klebsiella pneumoniae Carbapenemase in Enterobacteriaeeae[J]. J Clini Mierobiol, 2007, 45 (8) :2723-2725. 被引量:1
  • 2Justin MC, Audrey NS, Charles EH, et al. Development and evaluation of a real-time PCR assay for Detection of Klebsiella pneumonlae carbapenemase genes[J]. J Clini Microbiol, 2009,47(2) :322-326. 被引量:1
  • 3Wolter D J, Kurpiel PM, Woodford N,et al. Phenotypic and enzymatic comparative analysis of the novel KPC variant KPC-5 and its evolutionary variants, KPC-2 and KPC-4[J]. Antimicrob Agents Chemother, 2009,53(2) : 557-562. 被引量:1
  • 4Wei ZQ, Du XX, Yu YS, et al. Plasrnid mediated KPC-2 in a Klebsiella pneumoniae isolate from China [J]. Antimicrob A- gents Chemother, 2007,51:763-765. 被引量:1
  • 5Cai JC, Zhou HW, Zhang R, et al. Emergence of Serratia marcescens , Klebsiella pneumoniae , and Escherichia coli isolates possessing the plasmid-mediated carbapenem-hydrolyzing β-actamase KPC-2 in intensive care units of a Chinese hospital[J]. Antimicrob Agents Chemother, 2008,2(6): 2014-2018. 被引量:1
  • 6Marchaim D, Navon-Venezia S, Mitchell JS, et al. Isolation of irnipenem-resistant Enterobacter species: emergence of KPC-2 carbapenernase, molecular characterization, epiderniology, and outcomes[J]. Antirnierob Agents Chemother, 2008, 52 (4):1413-1418. 被引量:1
  • 7Tenover FC, KalsiRK, Williams PP,et al. Carbapenem resist ance in Klebsiella pneumoniae not detected by automated sus ceptibility testing[J]. Emerg Infect Dis, 2006, 12(8) : 1209- 1213. 被引量:1
  • 8Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing[-S] . 18th informational supplement. Approved standard M100-S18. CLSI,2008. 被引量:1
  • 9Zhang R,Yang LJ,Cai JC,et al. High-levelearbapenem resistance in a Citrobacter freundil clinical isolate is due to a combination of KPC-2 production and decreased porin expression[J]. J Med Microbiol, 2008,57 :332-337. 被引量:1
  • 10杨青,杜小幸,俞云松,李兰娟.5种药敏方法检测产KPC酶肠杆菌科细菌对碳青霉烯类抗生素的耐药性[J].浙江检验医学,2008,0(4):3-6. 被引量:14

共引文献6

同被引文献35

  • 1Yong D,Toleman MA,Giske CG,et al.Characterization of a new metallo-beta-lactamase gene,blaNDM-l,and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14from India[J].Antimicrob Agents Chemother,2009,53(12):5046-5054. 被引量:1
  • 2Huang TW,Chen TL,Chen YT,et al.Copy number change of the NDM-1sequence in a multidrug-resistant Klebsiella pneumoniae clinical isolate[J].PLoS One,2013,8:e62774. 被引量:1
  • 3Escobar Perez JAI,Olarte Escobar NM,Castro-Cardozo B,et al.Outbreak of NDM-1-producing Klebsiella pneumoniae in a neonatal unit in Colombia[J].Antimicrob Agents Chemother,2013,57(4):1957-1960. 被引量:1
  • 4Williamson DA,Sidjabat HE,Freeman JT,et al.Identification and molecular characterisation of New Delhi metallo-beta-lactamase-1(NDM-l)-and NDM-6-producing Enterobacteriaceae from New Zealand hospitals[J].Int J Antimicrob Agents,2012,39(6):529-533. 被引量:1
  • 5Zhao JY,Zhu YQ,Li YN,et al.Coexistence of SFO-1and NDM-1β-lactamase genes and fosfomycin resistance gene fosA3in an Escherichia coli clinical isolate[J].FEMS Microbiol Lett,2015,362(1):1-7. 被引量:1
  • 6陈萍(1962-),女(汉族),重庆市人,副主任技师,主要从事医院感染流行病学研究。mail:dpcpcq@sina.com陈萍,刘丁,陈萍.中国近30年医院感染暴发事件的流行特征与对策[J].中国感染控制杂志,2010,9(6):387-392. 被引量:38
  • 7刘运喜,刘丽娟,索继江,邢玉斌,贾宁,刘刚,肖红菊,高岩,邓春艳,杜明梅,曹务春.一起老年病区诺如病毒医院感染暴发的调查[J].中华医院感染学杂志,2011,21(6):1136-1138. 被引量:12
  • 8路超,王长印,董振芳,张建,邵松,卢志明.环介导等温扩增技术的应用[J].分子诊断与治疗杂志,2011,3(2):138-144. 被引量:18
  • 9林华胜,张薇,李国明.肺炎克雷伯菌耐药机制的研究进展[J].中国医学创新,2014,11(12):151-154. 被引量:14
  • 10张丽,朱元祺,张小兵,朱学海,张丽华,朱凯欣,周静,油丽萍,徐茶,赵静宜.耐碳青霉烯类肺炎克雷伯菌的耐药基因检测[J].中华医院感染学杂志,2014,24(23):5734-5736. 被引量:15

引证文献4

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈