期刊文献+

无花果果实韧皮部卸载路径由共质体向质外体途径转变 被引量:3

A shift of phloem unloading from symplasmic to apoplasmic pathway during fig fruit development
收藏 分享 导出
摘要 为了揭示无花果果实同化物韧皮部卸载机制,以玛斯义陶芬无花果5个时期果实为试材利用透射电子显微镜结合荧光染料示踪技术研究同化物韧皮部卸载路径的变化.无花果果实发育分3个时期,第1个快速生长期、缓慢生长期、第2个快速生长期,第2个生长期和第3个生长期之间为果实发育的转折期生长期之间.超微结构观察表明,无花果始熟期前筛分子伴胞复合体和周围薄壁细胞存在共质体联系,但始熟期后变为共质体隔离,同时在果实整个发育过程中薄壁细胞间始终存在大量胞间连丝.羧基荧光素(CF)引入表明,始熟期前荧光染料从韧皮部卸出到周围薄壁细胞中,在始熟期后CF被严格限制在韧皮部没有卸出到周围薄壁细胞.结果表明,无花果果实始熟期是同化物韧皮部卸载由共质体向质外体转变的转折期,但同时韧皮部后运输始终为共质体途径. To uncover the phloem unloading pathway of fig fruit, five-stage fruit of Masui Dauphine fig (Ficuscarica L., Moraceae) were used to research, including small green, big green, initial red, partial red, and full red, at about 15, 30, 65, 70 and 85 d after flower bud differentiation. A combination of electron microscopy and phloem-mobile symplasmic tracer carboxyfluorescein (CF), the phloem unloading pathway was studied in the developmental fruit, meanwhile, fruit size and color, as well as soluble sugar and starch contents were also analasis. The growth of fig fruit follows a typical sigmoid growth curve with distinct three developmental stages: stage I, the first rapid growth phase; stage II, the lag phase; stage III, the second rapid growth phase, namely, turning and ripening stages; the transition from stage II to stage III represents the onset of ripening. During stage I and stage II, fruit soluble sugar contents is lower, while the onset of the fruit ripening, a rapid increase in both fruit volume and soluble sugar contents with main glucose and fructose was observed. The contents of starch were decreasing rapidly before the onset of ripening, then after, decreased slowly. Electron microscopy observations showed that the sieve element-companion cell (SE-CC) complex of the vascular bundles is apparently symplasmically connected through plasmodesmata with surrounding parenchyma cells (PCs) during the stage I and stage II, but a transition occurred from symplastic to apoplastic after the onset of the fruit ripening. Interestingly, an abundance of plasmodesmata was always observed between parenchyma cells during the fruit development. Imaging of carboxyfluorescein movement showed that the dye is released from the functional phloem strands before the onset of ripening, whereas the CF was confined to the phloem strands after the onset of ripening, considering that a transient and critical developmental checkpoint is present around the onset of ripen- ing about 1-2 days, and the apoplasmic unload
作者 李春丽 侯柄竹 张晓燕 李疆 沈元月 LI ChunLi, HOU BingZhu, ZHANG XiaoYan, LI Jiang, SHEN YuanYue(1 College of Forest Science and Horticulture, Xinjiang Agriculural Univerity, Urumqi 830052, China;2 Beijing Key Laboratory of New Technology in Agricultural Application, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China; 3 College of Biology, China Agriculural Univerity, Beijing 100094, China)
出处 《科学通报》 EI CAS CSCD 北大核心 2016年第8期835-843,共9页 Chinese Science Bulletin
基金 国家自然科学基金(31471837) 北京市属高等学校创新团队建设与教师职业发展计划(IDHT20140509)资助
关键词 无花果 韧皮部卸载 超微结构 荧光示踪 共质体 质外体 fig fruit, phloem unloading, ultrastructure, carboxyfluorescein imaging, symplasmic, apoplasmic
作者简介 联系人,E—mail:sfmn@tom.com
  • 相关文献

参考文献27

  • 1Kislev M E, Anat H, Ofer B Y. Early domesticated fig in the Jordan Valley. Science, 2006, 312:1372-1374. 被引量:1
  • 2Stover E, Aradhya M, Ferguson L, et al. The fig:Overview of an ancient fruit. Hort Sci, 2007, 42:1083-1087. 被引量:1
  • 3Oparka K J. What is phloem unloading? Plant Physiol, 1990, 94:393-396. 被引量:1
  • 4Patrick J W. Phloem unloading:Sieve element unloading and post-sieve element transport. Plant Physiol, 1997, 48:191-222. 被引量:1
  • 5Patrick J W, Offler C E. Post-sieve element transport of photoassimilates in sink regions. J Exp Bot, 1996, 47:1165-1177. 被引量:1
  • 6Sj?lund R D. The phloem sieve element:A river runs through it. Plant Cell, 1997, 9:273-274. 被引量:1
  • 7Giaquinta R T, Lin W, Sadler N L, et al. Pathway of phloem unloading of sucrose in corn roots. Plant Physiol, 1983, 72:362-367. 被引量:1
  • 8Oparka K J, Duckett C M, Prior D A M, et al. Real-time imaging of phloem unloading in the root tip of Arabidopsis. Plant J, 2003, 6:759-766. 被引量:1
  • 9Liesche J, Martens H J, Schulz A. Symplasmic transport and phloem loading in gymnosperm leaves. Protoplasma, 2011, 248:181-190. 被引量:1
  • 10Viola R, Roberts A G, Haupt S, et al. Tuberization in potato involves a switch from apoplastic to symplastic phloem unloading. Plant Cell, 2001, 13:385-398. 被引量:1

二级参考文献18

  • 1李莉,杨雷,杨莉,郝保春.草莓果实生长发育及主要营养成分变化规律研究[J].江西农业学报,2006,18(2):67-70. 被引量:17
  • 2谢鸣,陈俊伟,秦巧平,蒋桂华,孙崇波,张慧琴,徐红霞.转化酶和己糖激酶调控草莓聚合果内糖积累[J].植物生理与分子生物学学报,2007,33(3):213-218. 被引量:14
  • 3马凯.无花果栽培与利用[M].南京:南京大学出版社,1992.8-80. 被引量:10
  • 4ALEXANDER L, GRIERSON D. Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening[J]. J Exp Bot, 2002, 53: 2039-2055. 被引量:1
  • 5PERKINS-VEAZIE P. Growth and ripening of strawberry fruit [J]. Hort Rev, 1995,17: 287-297. 被引量:1
  • 6FAIT A, HANHINEVA K, BELEGGIA R, DAI N, ROGACHEV I, NIKIFOROVA V J, FERNIE A R, AHARONI A. Reconfiguration of the achene and receptacle metabolic networks during strawberry fruit development[J]. Plant Physiol, 2008, 148(2): 730-750. 被引量:1
  • 7FINKELSTEIN R, GAMPALA S, ROCK C. Abscisic acid signaling in seeds and seedlings[J]. Plant Cell, 2002, 4: S15-S45. 被引量:1
  • 8SINGH M, LEWIS P E, HARDEMAN K, BAI L, ROSE J K, MA- ZOUREK M, CHOMET P, BRUTNELL T P. Activator mutagenesis of the pink scutelluml/viviparous7 locus of maize[J]. Plant Cell, 2003. 15: 874-884. 被引量:1
  • 9STURM A ,TANG G Q. The sucrose-cleaving enzymes of plants are crucial for development, growth and carbon partitionin[J]. Trends in Plant Sci, 1999(4): 401-407. 被引量:1
  • 10YAMAKI S. Physiology and metabolism of fruit development biochemistry of sugar metabolism and compartmentation in fruits[J]. Acta Hort, 1995, 398: 109-120. 被引量:1

共引文献17

同被引文献45

引证文献3

二级引证文献11

投稿分析

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈