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Tim-3和PD-1在慢性丙型肝炎患者不同单核细胞亚群的表达水平分析 被引量:4

Changes of Tim-3 and PD-1 on peripheral blood monocyte subsets in patients with chronic hepatitis C
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摘要 目的 分析慢性丙型肝炎(CHC)患者外周血单核细胞亚群分布,同时观察负性调节分子T细胞免疫球蛋白及黏蛋白域蛋白3(Tim-3)和程序性细胞死亡蛋白1(PD-1)在各亚群表达的变化及意义。方法 利用流式细胞术检测CHC患者外周血单核细胞亚群比例和各亚群Tim-3、PD-1表达,并与临床指标如丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)采用Spearman方法进行相关性分析。结果 与健康对照组比较,CHC患者外周血CD14+CD16+单核细胞比例增高,以CD14CD16+单核细胞比例增高显著。Tim-3在CD14CD16-单核细胞和CD14+CD16单核细胞上表达水平升高;PD-1在CD14CD16-单核细胞和CD14CD16+单核细胞上表达水平升高。CHC患者单核细胞亚群以及各亚群Tim-3、PD-1和临床指标ALT及AST无明显相关性。结论 Tim-3和PD-1在不同单核细胞亚群表达水平不同。 Objective To investigate the distribution of peripheral blood monocyte subsets of chronic hepatitis C (CHC) patients and observe the expression of negative regulators T cell immunoglobulin and mucin domain-3 (Tim-3) and programmed cell death-1 (PD-1) on the monocyte subsets. Methods Flow cytometry was employed to determine the distribution of three monocyte subsets as well as Tim-3 and PD-1 expression on the three monocyte subsets. Their correlations with the clinical parameters were analyzed by Spearman test. Results Compared with healthy controls, an increased distribution of CD14+ CD16+monocytes, especially CDI4++ CD16+ monocyte subset, was observed in CHC patients. Tim-3 expression was significantly elevated on CD14++CD16+ and CDI4 + CD16++ subsets in CHC patients. Obviously increased PD-1 expression was found mainly on CD14++ CD16+and CD14++ CD16+ subsets. There were no significant correlations between monocyte subsets, PD-1, Tim-3 and the clinical parameters. Conclusion The levels Tim-3 and PD-1 are different in three monocyte subsets.
作者 梁彦 张沛欣 伊文静 周云 贾战生 张颖 LIANG Yan, ZHANG Peixin, YI Wenjing, ZHOU Yun, JIA Zhansheng, ZHANG Ying( Department of Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China)
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2016年第5期666-670,共5页 Journal of Cellular and Molecular Immunology
基金 国家自然科学基金面上项目(81270499)
关键词 慢性丙型肝炎 单核细胞亚群 T细胞免疫球蛋白及黏蛋白域蛋白3(Tim-3) 程序性细胞死亡蛋白1(PD-1) chronic hepatitis C monocyte subsets T cell immunoglobulin and mucin domain-3 (Tim-3) programmed cell death-1 (PD-1)
作者简介 梁彦(1983-),女,陕西富平人,医师。硕士研究生Tel:13259752646;E-mail:hletian51@163.com. 通讯作者,张颖,E-mail:zyfmmu@hotmail.com
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