Objective: To develop the mice model of colorectal tumor-bearing with syndrome of heat-toxicity and blood stasis and observe the effects of activating blood and resolving stasis therapy. Methods: Establishing model by LPS of tail intravenous injection and peritoneal injection and C26 tumor cells subcutaneous injection, mice were randomly divided into 6 groups, respectively called blank control group(group A), tumor-bearing mice group(group B), control group with tumor(group C), tumor-bearing mice group with high dose herb medicine(group D), tumor-bearing mice group with middle dose herb medicine(group E), tumor-bearing mice group with low dose herb medicine(group F). After drug administration, quantified scoring, local microcirculation blood flow and hemorheology indices before and after molding were set up. Results: At the first week, mice in group of disease with TCM zheng showed different kinds of rise in temperature as well as vein stasis syndrome, mice in group D and group E had a gradually decreased score from molding 12 days to 42 days(P<0.05, P<0.01). After molding, regional blood flow of mice in the groups of disease integrating with TCM zheng reduced, after administration, regional blood flow of mice in these groups increased, especially in group D. After molding, there was significant increasing in the whole blood viscosity. Activating blood circulation can improve the state of hypercoagulability of blood to some extent. Conclusion: In this current study, we successfully developed the mice model of colorectal tumor-bearing with syndrome of heat-toxicity and blood stasis that well conformed to the TCM syndrome characteristics. In addition, the treatment of herbs with activating blood and resolving stasis at different doses improved the symptoms and signs to some extent.
China Journal of Traditional Chinese Medicine and Pharmacy
Animal model integrated with disease and syndrome
Cancer of colon
Syndrome of heat-toxicity and blood stasis