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糯玉米‘沪五彩花糯1号’品系特异性2b-RAD分子标记的开发及应用 被引量:3

Development and application of molecular markers for event-specific identification of waxy corn ‘Huwucaihuanuo 1' based on 2b-RAD technique
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摘要 应用基于IIB型限制性内切酶的RAD(2b-RAD)技术,对‘沪五彩花糯1号’及其亲本材料进行了简并基因组测序。利用RADtyping分型策略,通过与‘B73’参考基因组比对,我们在3个玉米样品的共同测序标签内筛选获得了9 726个单核苷酸多态性(SNP)位点。在此基础上,利用9 707个位于玉米染色体上的SNP位点对‘沪五彩花糯1号’及其亲本进行了基因型分析,开发出可用于‘沪五彩花糯1号’品系特异性鉴定的9个SNP分子标记。这些研究结果为‘沪五彩花糯1号’品系特异性鉴定和优质性状遗传组成的分子机理研究奠定了基础。 In this study,based on the next-generation sequencing data of ‘Huwucaihuanuo 1' and its parents obtained by type IIB endonucleases RAD(2b-RAD) technology,we identified 9 726 single nucleotide polymorphisms(SNPs) compared to that of ‘B73' reference genome.Among them,9 707 SNPs were located to chromosomes and were used for genotyping analysis of ‘Huwucaihuanuo 1' and its parents.Furthermore,we selected 9 SNPs to do molecular identification for ‘Huwucaihuanuo' 1.These results therefore provide basis for genotyping,molecular identification and genetic exploiting of ‘Huwucaihuanuo 1' in the future.
作者 李全林 王义发 韩晴 袁政 沈雪芳 LI Quan-Lin;WANG Yi-Fa;HAN Qing;YUAN Zheng;SHEN Xue-Fang( 1School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China; 2Shanghai Academy of Ag- ricultural Sciences, Shanghai 201403, China)
出处 《植物生理学报》 CAS CSCD 北大核心 2016年第5期669-677,共9页 Plant Physiology Communications
基金 上海市种业发展项目[沪农科种字(2013)第13号和沪农科推字(2014)第1-3号] 上海市科委基础研究重大重点项目(14JC1403901)
关键词 '沪五彩花糯1号’ 基因型 2b-RAD 品系特异性 SNP ‘Huwucaihuanuo 1' genotyping 2b-RAD event specificity SNP
作者简介 通讯作者(E-mail:zyuan@sjtu.edu.cn)。 通讯作者(E-mail:shenxuE-fang68@163.com)。
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  • 1Catchen JM, Amores A, Hohenlohe P, Cresko W, Postlethwait JH (2011). Stacks: building and genotyping loci de novo from short- read sequences. G3-Genes Genomes Genet, 1 (3): 171-182. 被引量:1
  • 2Ching A, Caldwell KS, Jung M, Dolan M, Smith OS, Tingey S, Mor- gante M, Rafalski AJ (2002). SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines. BMC Genet, 3:19. 被引量:1
  • 3Davey JW, Blaxter ML (2011). RADSeq: next-generation population genetics. Brief Funct Genomies, 9 (5): 416-423. 被引量:1
  • 4FedoroffN, Wessler S, Shure M (1983). Isolation of the transposable maize controlling elements A~ and Ds. Cell, 35 (1): 235-242. 被引量:1
  • 5Fletcher RS, Mullen JL, Yoder S, Bauerle WL, Reuning G, Sen S, Meyer E, Juenger TE, McKay JK (2013). Development of a next-generation NIL library in Arabidopsis thaliana for dissect- ing complex traits. BMC Genomies, 14:655. 被引量:1
  • 6Fu X, Dou J, Mao J, Su H, .liao W, Zhang L, Hu X, Huang X, Wang S, Bao Z (2013). RADtyping: an integrated package for accurate de novo eodominant and dominant RAD genotyping in mapping populations. PLoS ONE, 8 (11): e79960. 被引量:1
  • 7Guo Y, Yuan H, Fang D, Song L, Liu Y, Liu Y, Wu L, Yu J, Li Z, Xu X, et al (2014). An improved 2b-RAD approach (I2b-RAD) offer-ing genotyping tested by a rice (Oryza sativa L.) F2 population. BMC Genomics, 15 (1): 956. 被引量:1
  • 8Jiao W, Fu X, Dou J, Li H, Su H, Mao J, Yu Q, Zhang L, Hu X, Huang X, et al (2014). High-resolution linkage and quantitative trait locus n~apping aided by genome survey sequencing" buil, ding up an integrative genomic framework for a bivalve mollusc. DNA Res, 21 (1): 85-101. 被引量:1
  • 9Li C, Li Y, Bradbury PJ, Wu X, Shi ~, Song Y, Zhang D, Rodg- ers-Melnick E, Buckler ES, Zhang Z, et al (2015). Construction of high-quality recombination maps with low-coverage genomic sequencing for joint linkage analysis in maize. BMC Biol, 13:78. 被引量:1
  • 10Li R, Yu C, Li Y, Lam TW, Yiu SM, Kristiansen K, Wang J (2009). SOAP2: an improved ultrafast tool for short read alignment. Bioinformatics, 25 (15): 1966-1967. 被引量:1

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