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左归丸对去卵巢致绝经后骨质疏松症大鼠骨组织中Notch1、BMP9表达的影响 被引量:6

Influence of Zuogui Pill on expression of Notch1 and BMP9 in bone tissue of osteoporosis rats due to menopause after ovaries removal
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摘要 目的:通过研究去卵巢骨质疏松症(OP)大鼠股骨中Notch1和骨形态发生蛋白9(BMP9)的蛋白表达,探讨摘除卵巢致肾虚OP的病理机制,并研究左归丸的作用机制。方法:摘除雌性大鼠双侧卵巢的方法复制OP模型,采用左归丸对实验大鼠治疗12周,以福善美片作为阳性对照组,正常组、假手术组作为标准对照组,模型组作为空白对照,用ELISA法检测OP大鼠股骨Notch1和BMP9的蛋白表达。结果:正常大鼠股骨中存在Notch1和BMP9的蛋白表达。与正常组比较,模型组大鼠股骨中Notch1和BMP9的蛋白表达水平有明显的下降。左归丸组、福善美组用药12周后,与模型组相比较,左归丸组可显著上调股骨中Notch1和BMP9的蛋白表达水平(P〈0.01)。结论:股骨中Notch1和BMP9的蛋白表达下降可能是导致OP发生的机制之一;左归丸能够上调Notch1和BMP9的蛋白表达,表明左归丸对OP有一定的防治作用。 Objective: To explore the pathogenesis mechanism of osteoporosis due to menopause after ovaries removal, and study the action mechanism of Zuogui Pill by studying the expression of Notch1 and BMP9 in thighbone of osteoporosis rats due to menopause after ovaries removal. Methods: The osteoporosis rat models were established by removing bilateral ovaries of femal rats. Rats in experiment group were treated with Zuogui Pill for 12 weeks. Rats in positive group were treated with Fosamax. Rats in normal control group, sham operation group and model group were not treated. The expression of Notch1 and BMP9 proteins were tested by using ELISA method. Results: The results of ELISA showed that the expression of Notch1 and BMP9 in thighbone of rats in model group was significantly lower than that of normal control group. After treating with Fosamax and Zuogui Pill for 12 weeks, Zuogui Pill could increase the expression of Notch1 and BMP9 in thighbone of rats(P0.01). Conclusion: The decrease in expression of Notch1 and BMP9 proteins might be one of the mechanism of osteoporosis. Zuogui Pill could increase the expression of Notch1 and BMP9 proteins, which showed some prevention and curative effects on osteoporosis.
作者 周强 孙鑫 邓洋洋 姜坤 邢克欣 林庶茹 朱嘉绮 尚德阳 ZHOU Qiang, SUN Xin, DENG Yang-yang, JIANG Kun, XING Ke-xin, LIN Shu-ru, ZHU Jia-qi, SHANG De-yang ( Liaoning University of TCM, Shenyang 110032, China )
机构地区 辽宁中医药大学
出处 《中华中医药杂志》 CAS CSCD 北大核心 2016年第7期2782-2784,共3页 China Journal of Traditional Chinese Medicine and Pharmacy
基金 国家自然科学基金项目(No.81273618) 国家自然科学基金青年基金项目(No.81302879) 辽宁省自然基金项目(No.20125004)
关键词 肾虚 骨质疏松症 NOTCH1 骨形成发生蛋白9 左归丸 Kidney deficiency Osteoporosis Notch1 Bone morphogenetic protein 9(BMP9) Zuogui Pill
作者简介 通讯作者:尚德阳,沈阳市皇姑区崇山东路79号辽宁中医药大学基础医学院,邮编:110032,电话:024—31207077E—mail:shangdeyang@sohu.com
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