The canine arvovirus （CPV） isolated from dog feces was cultured and purified. SP2/0 myeloma cells SP2/0 were fused with spleen cells from CPV immunized Balb/c mice. Positive cells producing antibodies were screened by indirect ELISA,and a monoclonal antibody against CPV was generated and designated as 3E2. The monoelonal antibody belongs to IgG1. The titer of aseites was 1.8 × 10^5 as detected by ELISA. No cross-reactions were observed between the McAb prepared and other canine viruses and the McAb could recognize CPV. Based on the McAb, the GICA was established by purified and gold labeled McAb 3E2. It could detect the CPV specifically. Therefore, the results indicated that the method had high specificity. It could serve as effective detection measure for clinic and further research of CPV.
Progress In Veterinary Medicine