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浒苔多糖的酶法降解及其工艺条件优化的研究 预览 被引量:5

Study on enzymatic degradation for polysaccharide from Enteromorpha and its technical condition optimization
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摘要 采用生物酶法将浒苔多糖降解为分子质量较小、粘度较低的浒苔寡糖,以还原糖生成量作为指标,通过单因素和响应面法优化降解工艺,利用液相色谱和质谱技术对酶解产物进行了组分分析。结果表明:浒苔多糖降解酶能够实现对浒苔多糖的高效降解,最适酶解条件为加酶量1.5%、底物质量浓度14 mg/m L、初始p H=6.5、反应时间6 h、温度33℃,在此条件下,还原糖生成量为165.21 mg,降解率为61.21%,粘度由349.89 m Pa·s降低至2.05 m Pa·s。单糖组成结果表明酶解产物由鼠李糖、葡萄糖醛酸、葡萄糖、木糖组成,质谱分析表明酶解产物包括酸性寡糖片段和中性寡糖片段。 The purpose of this study was to hydrolyze PE for oligosaccharide using specific PE degradase. The enzymatic hydrolysis conditions were optimized. The saccharide composition of the hydrolysate fractions was analyzed by liquid chromatography and electrospray ionization mass spectrometry.The yield of reducing sugar was chosen as an evaluating indicator during single factor experiments and response surface methodology. Results showed that the enzyme could hydrolyze PE efficiently.The optimal hydrolysis condition was as follows, enzyme dosage 1.5%, substrate concentration 14 mg/mL,the initial pH6.5, reaction time 6 h, and hydrolysis temperature of 33 ℃.The corresponding reducing sugar yield and hydrolysis efficiency was 165.21 mg and 61.21%, respectively. Besides,the viscosity of PE reduced from 349.89 mPa. s to 2.05 mPa. s.The HPLC results indicated that the monosaccharide composition of the hydrolysate was rhamnose,glucuronic acid,glucose and xylose.The results of mass analysis showed that degradase could degrade sulfate PE and neutral PE.
作者 张高丽 李银平 赵梅 刘盟梦 王鹏 ZHANG Gao- li, LI Yin- ping, ZHAO Mei, LIU Meng- meng, WANG Peng (College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China)
出处 《食品工业科技》 CAS CSCD 北大核心 2016年第18期202-207,共6页 Science and Technology of Food Industry
基金 山东省自然科学基金(ZR2015DQ005) 青岛市应用基础研究计划(15-9-1-62-jch).
关键词 浒苔多糖 降解酶 响应面 粘度 酶解工艺 polysaccharides of Enteromorpha degrading enzyme response surface methodology viscosityoptimization of enzymatic hydrolysis
作者简介 张高丽(1990-),女,硕士,研究方向:食品加工与功能食品,E-mail:sucigel@163.com 通讯作者:王鹏(1980-),男,副教授,研究方向:海洋应用微生物,E-mail:pengwang@ouc.edu.cn
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